Abstract
The 5' terminus of each nascent daughter strand of adenovirus DNA replicated in vitro is covalently linked to a protein with an apparent Mr of 80,000. This protein may represent a precursor to the 55,000-dalton protein known to be linked to the 5' ends of mature adenovirus DNA strands. Partial proteolysis experiments indicate that the 80,000-dalton and 55,000-dalton proteins are structurally related. Furthermore, both proteins are attached to DNA by the same linkage: a phosphodiester bond between the beta-OH of a serine residue in the protein and the 5'-OH of the terminal deoxycytidine residue of the DNA. The role of the 80,000-dalton protein in adenovirus DNA replication is not yet clear, although one reasonable possibility is that it serves as the primer for daughter strand synthesis.
Dates
| Type | When |
|---|---|
| Created | 19 years, 3 months ago (May 31, 2006, 4:27 a.m.) |
| Deposited | 3 years, 4 months ago (April 13, 2022, 11:28 a.m.) |
| Indexed | 4 months, 3 weeks ago (April 12, 2025, 9:38 p.m.) |
| Issued | 45 years ago (Sept. 1, 1980) |
| Published | 45 years ago (Sept. 1, 1980) |
| Published Online | 45 years ago (Sept. 1, 1980) |
| Published Print | 45 years ago (Sept. 1, 1980) |
@article{Challberg_1980, title={Adenovirus DNA replication in vitro: characterization of a protein covalently linked to nascent DNA strands.}, volume={77}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.77.9.5105}, DOI={10.1073/pnas.77.9.5105}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Challberg, M D and Desiderio, S V and Kelly, T J}, year={1980}, month=sep, pages={5105–5109} }