DOI: 10.1073/pnas.77.8.4679. Chemical probes for higher-order structure in RNA.

Chemical probes for higher-order structure in RNA.

10.1073/pnas.77.8.4679

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Three chemical reactions can probe the secondary and tertiary interactions of RNA molecules in solution. Dimethyl sulfate monitors the N-7 of guanosines and senses tertiary interactions there, diethyl pyrocarbonate detects stacking of adenosines, and an alternate dimethyl sulfate reaction examines the N-3 of cytidines and thus probes base pairing. The reactions work between 0 degrees C and 90 degrees C and at pH 4.5--8.5 in a variety of buffers. As an example we follow the progressive denaturation of yeast tRNAPhe terminally labeled with 32P as the tertiary and secondary structures sequentially melt out. A single autoradiograph of a terminally labeled molecule locates regions of higher-order structure and identifies the bases involved.

Bibliography

Peattie, D. A., & Gilbert, W. (1980). Chemical probes for higher-order structure in RNA. Proceedings of the National Academy of Sciences, 77(8), 4679â4682.

Authors 2

D A Peattie (first)
W Gilbert (additional)

References 0 Referenced 281

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 4:26 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:21 a.m.)
Indexed	1 day, 14 hours ago (Aug. 30, 2025, 1:03 p.m.)
Issued	45 years, 1 month ago (Aug. 1, 1980)
Published	45 years, 1 month ago (Aug. 1, 1980)
Published Online	45 years, 1 month ago (Aug. 1, 1980)
Published Print	45 years, 1 month ago (Aug. 1, 1980)

Funders 0

None

BibTeX

@article{Peattie_1980, title={Chemical probes for higher-order structure in RNA.}, volume={77}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.77.8.4679}, DOI={10.1073/pnas.77.8.4679}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Peattie, D A and Gilbert, W}, year={1980}, month=aug, pages={4679–4682} }

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