DOI: 10.1073/pnas.77.8.4669. Procoat, the precursor of M13 coat protein, requires an electrochemical potential for membrane insertion.

Procoat, the precursor of M13 coat protein, requires an electrochemical potential for membrane insertion.

10.1073/pnas.77.8.4669

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The coat protein of coliphage M13 spans the host cell cytoplasmic membrane prior to its assembly into extruding virus. It is made as a soluble cytoplasmic precursor, termed "procoat," with 23 extra amino acid residues at the NH2 terminus. Procoat binds to the cell membrane and is converted proteolytically to coat protein. When the electrochemical gradient of an infected cell is rapidly dissipated by uncouplers, procoat still binds to the plasma membrane but is not converted to coat. We report here that membrane-bound procoat is only detected at the inner face of the cytoplasmic membrane and that uncouplers prevent it from integrating into a transmembrane conformation.

Bibliography

Date, T., Goodman, J. M., & Wickner, W. T. (1980). Procoat, the precursor of M13 coat protein, requires an electrochemical potential for membrane insertion. Proceedings of the National Academy of Sciences, 77(8), 4669â4673.

Authors 3

T Date (first)
J M Goodman (additional)
W T Wickner (additional)

References 0 Referenced 97

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 4:26 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:20 a.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 4:30 p.m.)
Issued	45 years ago (Aug. 1, 1980)
Published	45 years ago (Aug. 1, 1980)
Published Online	45 years ago (Aug. 1, 1980)
Published Print	45 years ago (Aug. 1, 1980)

Funders 0

None

BibTeX

@article{Date_1980, title={Procoat, the precursor of M13 coat protein, requires an electrochemical potential for membrane insertion.}, volume={77}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.77.8.4669}, DOI={10.1073/pnas.77.8.4669}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Date, T and Goodman, J M and Wickner, W T}, year={1980}, month=aug, pages={4669–4673} }

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