DOI: 10.1073/pnas.77.6.3369. Eukaryotic signal sequence transports insulin antigen in Escherichia coli.

Eukaryotic signal sequence transports insulin antigen in Escherichia coli.

10.1073/pnas.77.6.3369

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We made a series of plasmids with unique Pst restriction sites within or near the DNA that encodes the penicillinase signal sequence. Inserted DNA can be read in all three frames both within and immediately after the signal sequence. We cloned Pst-terminated DNA copies of the structural information for rat proinsulin and preproinsulin into these plasmids, forming a large number of hybrid penicillinase (bacterial) and insulin (eukaryotic) signal sequences. We then compared the levels of insulin antigen in the Escherichia coli periplasm with those inside the cells. We conclude that either the bacterial or the eukaryotic signal is sufficient to transport rat insulin antigen into the periplasmic space.

Authors 3

K Talmadge (first)
S Stahl (additional)
W Gilbert (additional)

References 0 Referenced 123

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 4:21 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:18 a.m.)
Indexed	1 year ago (Aug. 10, 2024, 1:53 a.m.)
Issued	45 years, 2 months ago (June 1, 1980)
Published	45 years, 2 months ago (June 1, 1980)
Published Online	45 years, 2 months ago (June 1, 1980)
Published Print	45 years, 2 months ago (June 1, 1980)

Funders 0

None

BibTeX

@article{Talmadge_1980, title={Eukaryotic signal sequence transports insulin antigen in Escherichia coli.}, volume={77}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.77.6.3369}, DOI={10.1073/pnas.77.6.3369}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Talmadge, K and Stahl, S and Gilbert, W}, year={1980}, month=jun, pages={3369–3373} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        8,
        10
      ]
    ],
    "date-time": "2024-08-10T05:53:05Z",
    "timestamp": 1723269185158
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "6",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1980,
        6
      ]
    ]
  },
  "abstract": "<jats:p>We made a series of plasmids with unique Pst restriction sites within or near the DNA that encodes the penicillinase signal sequence. Inserted DNA can be read in all three frames both within and immediately after the signal sequence. We cloned Pst-terminated DNA copies of the structural information for rat proinsulin and preproinsulin into these plasmids, forming a large number of hybrid penicillinase (bacterial) and insulin (eukaryotic) signal sequences. We then compared the levels of insulin antigen in the Escherichia coli periplasm with those inside the cells. We conclude that either the bacterial or the eukaryotic signal is sufficient to transport rat insulin antigen into the periplasmic space.</jats:p>",
  "DOI": "10.1073/pnas.77.6.3369",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T08:21:20Z",
    "timestamp": 1149063680000
  },
  "page": "3369-3373",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 123,
  "title": "Eukaryotic signal sequence transports insulin antigen in Escherichia coli.",
  "prefix": "10.1073",
  "volume": "77",
  "author": [
    {
      "given": "K",
      "family": "Talmadge",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "S",
      "family": "Stahl",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "W",
      "family": "Gilbert",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1980,
        6
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.77.6.3369",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T15:18:15Z",
    "timestamp": 1649863095000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.77.6.3369"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1980,
        6
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          1980,
          6
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.77.6.3369"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.77.6.3369",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1980,
        6
      ]
    ]
  },
  "assertion": [
    {
      "value": "1980-06-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}