DOI: 10.1073/pnas.77.5.2611. Sequences of the recA gene and protein.

10.1073/pnas.77.5.2611

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have determined the nucleotide sequence of the recA gene of Escherichia coli; this permits the formulation of the primary structure for the recA protein. This structure is consistent with the amino acid composition of the tryptic peptides obtained from the recA protein. The coding region of the recA gene has 1059 base pairs, which specify 352 amino acids. The recA protein has alanine and phenylalanine as its NH2- and COOH-terminal amino acids, respectively, and has the following amino acid composition: Cys3 Asp20 Asn15 Met9 Thr17 Ser20 Glu30 Gln13 Pro10 Gly35 Ala38 Val22 Ile27 Leu31 Tyr7 Phe10 His2Lys27 Trp2 Arg14. Of the three cysteine residues, only two can be alkylated under reducing and denaturing conditions. The molecular weight of the recA polypeptide is 37,842.

Bibliography

Sancar, A., Stachelek, C., Konigsberg, W., & Rupp, W. D. (1980). Sequences of the recA gene and protein. Proceedings of the National Academy of Sciences, 77(5), 2611â2615.

Authors 4

A Sancar (first)
C Stachelek (additional)
W Konigsberg (additional)
W D Rupp (additional)

References 0 Referenced 173

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 4:13 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:31 a.m.)
Indexed	4 months, 2 weeks ago (April 8, 2025, 5:39 p.m.)
Issued	45 years, 3 months ago (May 1, 1980)
Published	45 years, 3 months ago (May 1, 1980)
Published Online	45 years, 3 months ago (May 1, 1980)
Published Print	45 years, 3 months ago (May 1, 1980)

Funders 0

None

BibTeX

@article{Sancar_1980, title={Sequences of the recA gene and protein.}, volume={77}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.77.5.2611}, DOI={10.1073/pnas.77.5.2611}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sancar, A and Stachelek, C and Konigsberg, W and Rupp, W D}, year={1980}, month=may, pages={2611–2615} }

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