DOI: 10.1073/pnas.77.3.1331. Supercoils in prokaryotic DNA restrained in vivo.

Supercoils in prokaryotic DNA restrained in vivo.

10.1073/pnas.77.3.1331

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Cells of Escherichia coli containing the plasmid F were gamma-irradiated with various doses to introduce determined numbers of single-strand breaks in the F DNA. The cells were then incubated to permit repair of the breaks while DNA gyrase was inhibited with coumermycin to limit restoration of any relaxed supercoil. Repaired, covalently continuous F DNA was isolated and its superhelical density was measured by two different methods. Both indicated that a major part (50-60%) of the negative superhelical turns were maintained in the repaired molecules, suggesting that the supercoils are restrained in vivo.

Bibliography

Pettijohn, D. E., & Pfenninger, O. (1980). Supercoils in prokaryotic DNA restrained in vivo. Proceedings of the National Academy of Sciences, 77(3), 1331â1335.

Authors 2

D E Pettijohn (first)
O Pfenninger (additional)

References 0 Referenced 95

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 4:11 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:18 a.m.)
Indexed	1 year ago (Aug. 7, 2024, 8:30 a.m.)
Issued	45 years, 5 months ago (March 1, 1980)
Published	45 years, 5 months ago (March 1, 1980)
Published Online	45 years, 5 months ago (March 1, 1980)
Published Print	45 years, 5 months ago (March 1, 1980)

Funders 0

None

BibTeX

@article{Pettijohn_1980, title={Supercoils in prokaryotic DNA restrained in vivo.}, volume={77}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.77.3.1331}, DOI={10.1073/pnas.77.3.1331}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Pettijohn, D E and Pfenninger, O}, year={1980}, month=mar, pages={1331–1335} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        8,
        7
      ]
    ],
    "date-time": "2024-08-07T12:30:28Z",
    "timestamp": 1723033828825
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "3",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1980,
        3
      ]
    ]
  },
  "abstract": "<jats:p>Cells of Escherichia coli containing the plasmid F were gamma-irradiated with various doses to introduce determined numbers of single-strand breaks in the F DNA. The cells were then incubated to permit repair of the breaks while DNA gyrase was inhibited with coumermycin to limit restoration of any relaxed supercoil. Repaired, covalently continuous F DNA was isolated and its superhelical density was measured by two different methods. Both indicated that a major part (50-60%) of the negative superhelical turns were maintained in the repaired molecules, suggesting that the supercoils are restrained in vivo.</jats:p>",
  "DOI": "10.1073/pnas.77.3.1331",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T08:11:35Z",
    "timestamp": 1149063095000
  },
  "page": "1331-1335",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 95,
  "title": "Supercoils in prokaryotic DNA restrained in vivo.",
  "prefix": "10.1073",
  "volume": "77",
  "author": [
    {
      "given": "D E",
      "family": "Pettijohn",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "O",
      "family": "Pfenninger",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1980,
        3
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.77.3.1331",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T15:18:30Z",
    "timestamp": 1649863110000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.77.3.1331"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1980,
        3
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1980,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.77.3.1331"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.77.3.1331",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1980,
        3
      ]
    ]
  },
  "assertion": [
    {
      "value": "1980-03-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}