DOI: 10.1073/pnas.76.9.4294. Structure of genes for human growth hormone and chorionic somatomammotropin.

Structure of genes for human growth hormone and chorionic somatomammotropin.

10.1073/pnas.76.9.4294

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A 2.6-kilobase (kb) EcoRI restriction endonuclease fragment containing human growth hormone (hGH; somatotropin) gene sequences and a 2.8-kb EcoRI fragment containing human chorionic somatomammotropin (hCS; choriomammotropin) gene sequences have been identified by hybridization to cloned cDNA. Human DNA was cleaved with EcoRI and fractionated by preparative agarose gel electrophoresis; DNA in the size range 2--3 kb was ligated to lambda gt WES.lambda B DNA and viable recombinant bacteriophage were recovered by in vitro packaging. After infection of Escherichia coli and screening of phage plaques, single isolates of hGH and hCS gene sequences were obtained. Restriction endonuclease mapping showed that the hGH gene contains three intervening sequences interrupting the coding sequence. Partial DNA sequence analysis of the hGH gene, obtained by the chain termination method, confirmed the location of the intervening sequences and the identity of the fragment.

Bibliography

Fiddes, J. C., Seeburg, P. H., DeNoto, F. M., Hallewell, R. A., Baxter, J. D., & Goodman, H. M. (1979). Structure of genes for human growth hormone and chorionic somatomammotropin. Proceedings of the National Academy of Sciences, 76(9), 4294â4298.

Authors 6

J C Fiddes (first)
P H Seeburg (additional)
F M DeNoto (additional)
R A Hallewell (additional)
J D Baxter (additional)
H M Goodman (additional)

References 0 Referenced 60

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 4:03 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:31 a.m.)
Indexed	4 months, 1 week ago (April 22, 2025, 4:03 p.m.)
Issued	45 years, 11 months ago (Sept. 1, 1979)
Published	45 years, 11 months ago (Sept. 1, 1979)
Published Online	45 years, 11 months ago (Sept. 1, 1979)
Published Print	45 years, 11 months ago (Sept. 1, 1979)

Funders 0

None

BibTeX

@article{Fiddes_1979, title={Structure of genes for human growth hormone and chorionic somatomammotropin.}, volume={76}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.76.9.4294}, DOI={10.1073/pnas.76.9.4294}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Fiddes, J C and Seeburg, P H and DeNoto, F M and Hallewell, R A and Baxter, J D and Goodman, H M}, year={1979}, month=sep, pages={4294–4298} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        4,
        22
      ]
    ],
    "date-time": "2025-04-22T20:03:34Z",
    "timestamp": 1745352214896
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "9",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1979,
        9
      ]
    ]
  },
  "abstract": "<jats:p>A 2.6-kilobase (kb) EcoRI restriction endonuclease fragment containing human growth hormone (hGH; somatotropin) gene sequences and a 2.8-kb EcoRI fragment containing human chorionic somatomammotropin (hCS; choriomammotropin) gene sequences have been identified by hybridization to cloned cDNA. Human DNA was cleaved with EcoRI and fractionated by preparative agarose gel electrophoresis; DNA in the size range 2--3 kb was ligated to lambda gt WES.lambda B DNA and viable recombinant bacteriophage were recovered by in vitro packaging. After infection of Escherichia coli and screening of phage plaques, single isolates of hGH and hCS gene sequences were obtained. Restriction endonuclease mapping showed that the hGH gene contains three intervening sequences interrupting the coding sequence. Partial DNA sequence analysis of the hGH gene, obtained by the chain termination method, confirmed the location of the intervening sequences and the identity of the fragment.</jats:p>",
  "DOI": "10.1073/pnas.76.9.4294",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T08:03:12Z",
    "timestamp": 1149062592000
  },
  "page": "4294-4298",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 60,
  "title": "Structure of genes for human growth hormone and chorionic somatomammotropin.",
  "prefix": "10.1073",
  "volume": "76",
  "author": [
    {
      "given": "J C",
      "family": "Fiddes",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "P H",
      "family": "Seeburg",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "F M",
      "family": "DeNoto",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "R A",
      "family": "Hallewell",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J D",
      "family": "Baxter",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "H M",
      "family": "Goodman",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1979,
        9
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.76.9.4294",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T15:31:25Z",
    "timestamp": 1649863885000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.76.9.4294"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1979,
        9
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "9",
    "published-print": {
      "date-parts": [
        [
          1979,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.76.9.4294"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.76.9.4294",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1979,
        9
      ]
    ]
  },
  "assertion": [
    {
      "value": "1979-09-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}