DOI: 10.1073/pnas.76.7.3174. Nucleoside triphosphate regeneration decreases the frequency of translation errors.

Nucleoside triphosphate regeneration decreases the frequency of translation errors.

10.1073/pnas.76.7.3174

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The addition of naturally occurring polyamines and inorganic ions to an in vitro protein-synthesizing system improved the extent and fidelity of translation. In such an optimized system, regeneration of the nucleoside triphosphates with phosphoenolpyruvate and pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) reduced further the missense error frequency to the in vivo level as well as enhanced the extent of translation. The effect of nucleoside triphosphate regeneration was shown to be due primarily to the increase in the ratio of adenosine and guanosine triphosphates to their hydrolysis products and only marginally due to the increase in the absolute concentrations of the nucleoside triphosphates.

Bibliography

Jelenc, P. C., & Kurland, C. G. (1979). Nucleoside triphosphate regeneration decreases the frequency of translation errors. Proceedings of the National Academy of Sciences, 76(7), 3174â3178.

Authors 2

P C Jelenc (first)
C G Kurland (additional)

References 0 Referenced 217

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 4:02 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:29 a.m.)
Indexed	1 year, 1 month ago (July 15, 2024, 10:11 p.m.)
Issued	46 years, 1 month ago (July 1, 1979)
Published	46 years, 1 month ago (July 1, 1979)
Published Online	46 years, 1 month ago (July 1, 1979)
Published Print	46 years, 1 month ago (July 1, 1979)

Funders 0

None

BibTeX

@article{Jelenc_1979, title={Nucleoside triphosphate regeneration decreases the frequency of translation errors.}, volume={76}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.76.7.3174}, DOI={10.1073/pnas.76.7.3174}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Jelenc, P C and Kurland, C G}, year={1979}, month=jul, pages={3174–3178} }

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