DOI: 10.1073/pnas.76.3.1373. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

10.1073/pnas.76.3.1373

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In this report, we demonstrate the feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase (aprt; AMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.7) to the aprt+ phenotype by means of DNA-mediated gene transfer. Transformation was effected by using unfractionated high molecular weight genomic DNA from Chinese hamster, human, and mouse cells and restriction endonuclease-digested DNA from rabbit liver. The transformation frequency observed was between 1 and 10 colonies per 10(6) cells per 20 microgram of donor DNA. Transformants displayed enzymatic activity that was donor derived as demonstrated by isoelectric focusing of cytoplasmic extracts. These transformants fall into two classes: those that are phenotypically stable when grown in the absence of selective pressure and those that are phenotypically unstable under the same conditions.

Bibliography

Wigler, M., Pellicer, A., Silverstein, S., Axel, R., Urlaub, G., & Chasin, L. (1979). DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells. Proceedings of the National Academy of Sciences, 76(3), 1373â1376.

Authors 6

M Wigler (first)
A Pellicer (additional)
S Silverstein (additional)
R Axel (additional)
G Urlaub (additional)
L Chasin (additional)

References 0 Referenced 746

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:57 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:21 a.m.)
Indexed	1 month, 2 weeks ago (July 12, 2025, 6:57 p.m.)
Issued	46 years, 5 months ago (March 1, 1979)
Published	46 years, 5 months ago (March 1, 1979)
Published Online	46 years, 5 months ago (March 1, 1979)
Published Print	46 years, 5 months ago (March 1, 1979)

Funders 0

None

BibTeX

@article{Wigler_1979, title={DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.}, volume={76}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.76.3.1373}, DOI={10.1073/pnas.76.3.1373}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wigler, M and Pellicer, A and Silverstein, S and Axel, R and Urlaub, G and Chasin, L}, year={1979}, month=mar, pages={1373–1376} }

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