DOI: 10.1073/pnas.76.3.1094. Control of eIF-2 phosphatase activity in rabbit reticulocyte lysate.

Control of eIF-2 phosphatase activity in rabbit reticulocyte lysate.

10.1073/pnas.76.3.1094

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A sensitive assay requiring picomole amounts of [32P]eIF-2 to measure eIF-2 phosphatase activity has been developed. Dephosphorylation of [32P]eIF-2 alpha (38,000-dalton subunit) is extremely rapid (t 1/2 = 20 sec) and occurs at the same rate in both hemin-supplemented and hemin-depleted lysates. In contrast, [32P]eIF-2 beta phosphate is stable under all conditions studied. At concentrations required to produce a transient inhibition of protein synthesis, GDP prevents dephosphorylation of half of the phosphate introduced on eIF-2 alpha by the hemin-controlled repressor. Equimolar GTP is without effect. The concept that the energy charge of the guanylate pool may regulate accessibility of a phosphorylated site on eIF-2 alpha to its phosphatase and the implication of this to the mechanism of hemin-regulated translational control are discussed.

Bibliography

Safer, B., & Jagus, R. (1979). Control of eIF-2 phosphatase activity in rabbit reticulocyte lysate. Proceedings of the National Academy of Sciences, 76(3), 1094â1098.

Authors 2

B Safer (first)
R Jagus (additional)

References 0 Referenced 43

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:57 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:52 a.m.)
Indexed	1 year, 1 month ago (July 19, 2024, 1:50 p.m.)
Issued	46 years, 5 months ago (March 1, 1979)
Published	46 years, 5 months ago (March 1, 1979)
Published Online	46 years, 5 months ago (March 1, 1979)
Published Print	46 years, 5 months ago (March 1, 1979)

Funders 0

None

BibTeX

@article{Safer_1979, title={Control of eIF-2 phosphatase activity in rabbit reticulocyte lysate.}, volume={76}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.76.3.1094}, DOI={10.1073/pnas.76.3.1094}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Safer, B and Jagus, R}, year={1979}, month=mar, pages={1094–1098} }

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