DOI: 10.1073/pnas.76.11.5728. Estimation of membrane potentials of individual lymphocytes by flow cytometry.

Estimation of membrane potentials of individual lymphocytes by flow cytometry.

10.1073/pnas.76.11.5728

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The membrane potentials of individual cells can be estimated by flow cytometric quantitation of the cells' uptake of the fluorescent lipophilic cationic dye 3,3'-dihexyloxacarbocyanine iodide. Human lymphocytes separated from peripheral blood on Hypaque-Ficoll gradients are uniformly depolarized by gramicidin and hyperpolarized by valinomycin. Concanavalin A and phytohemagglutinin depolarize only a fraction of the lymphocytes. The flow cytometric technique allows precise detection of heterogeneous membrane potential responses to stimuli such as lectins; it could also provide a basis for sorting cells that respond differently to a given stimulus.

Bibliography

Shapiro, H. M., Natale, P. J., & Kamentsky, L. A. (1979). Estimation of membrane potentials of individual lymphocytes by flow cytometry. Proceedings of the National Academy of Sciences, 76(11), 5728â5730.

Authors 3

H M Shapiro (first)
P J Natale (additional)
L A Kamentsky (additional)

References 0 Referenced 100

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:52 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:52 a.m.)
Indexed	1 week, 2 days ago (Aug. 27, 2025, 11:39 a.m.)
Issued	45 years, 10 months ago (Nov. 1, 1979)
Published	45 years, 10 months ago (Nov. 1, 1979)
Published Online	45 years, 10 months ago (Nov. 1, 1979)
Published Print	45 years, 10 months ago (Nov. 1, 1979)

Funders 0

None

BibTeX

@article{Shapiro_1979, title={Estimation of membrane potentials of individual lymphocytes by flow cytometry.}, volume={76}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.76.11.5728}, DOI={10.1073/pnas.76.11.5728}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Shapiro, H M and Natale, P J and Kamentsky, L A}, year={1979}, month=nov, pages={5728–5730} }

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