DOI: 10.1073/pnas.76.11.5529. Control of phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli by energization of the cell membrane.

Control of phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli by energization of the cell membrane.

10.1073/pnas.76.11.5529

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli is inhibited by the energized of the membrane. This was shown in intact cells as well as in membrane vesicles. Relaxation of the proton gradient by uncouplers stimulated the uptake of sugars via the phosphotransferase system in aerobically cultured cells. No such effect was seen in anaerobic cells, apparently because the cell membrane of these cells is poorly energized. Energization by respiration of D-lactate or ascorbate inhibited the phosphotransferase uptake system in membrane vesicles. This inhibition was reversed by the addition of cyanide. Oxamate, a specific inhibitor of lactate dehydrogenase, prevented the inhibitory effect of D-lactate. Membrane vesicles prepared from a cytochrome-less mutant were not energized by D-lactate oxidation and the phosphotransferase uptake system was not inhibited.

Bibliography

Reider, E., Wagner, E. F., & Schweiger, M. (1979). Control of phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli by energization of the cell membrane. Proceedings of the National Academy of Sciences, 76(11), 5529â5533.

Authors 3

E Reider (first)
E F Wagner (additional)
M Schweiger (additional)

References 0 Referenced 28

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:52 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:51 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 3:55 p.m.)
Issued	45 years, 10 months ago (Nov. 1, 1979)
Published	45 years, 10 months ago (Nov. 1, 1979)
Published Online	45 years, 10 months ago (Nov. 1, 1979)
Published Print	45 years, 10 months ago (Nov. 1, 1979)

Funders 0

None

BibTeX

@article{Reider_1979, title={Control of phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli by energization of the cell membrane.}, volume={76}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.76.11.5529}, DOI={10.1073/pnas.76.11.5529}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Reider, E and Wagner, E F and Schweiger, M}, year={1979}, month=nov, pages={5529–5533} }

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