Abstract
Oocytes of the frog Xenopus were injected with purified circular DNA containing amplified rRNA genes of the water beetle Dytiscus. Nuclear contents of injected oocytes were spread and examined by electron microscopy. Most of the Dytiscus DNA seen in injected nuclei contained regions indensely packed with polymerases and nascent transcripts. Apparently normal, as well as abnormal, patterns of transcription were observed. By this type of experiment, it may become possible to recognize the transcribed regions and immediate transcripts of cloned DNA molecules whose activity cannot be seen by electron microscopy of normal nuclei.
Dates
Type | When |
---|---|
Created | 19 years, 3 months ago (May 31, 2006, 3:47 a.m.) |
Deposited | 3 years, 4 months ago (April 13, 2022, 10:55 a.m.) |
Indexed | 1 year, 10 months ago (Oct. 31, 2023, 4:54 p.m.) |
Issued | 47 years, 1 month ago (Aug. 1, 1978) |
Published | 47 years, 1 month ago (Aug. 1, 1978) |
Published Online | 47 years, 1 month ago (Aug. 1, 1978) |
Published Print | 47 years, 1 month ago (Aug. 1, 1978) |
@article{Trendelenburg_1978, title={Transcription patterns of amplified Dytiscus genes coding for ribosomal RNA after injection into Xenopus oocyte nuclei.}, volume={75}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.75.8.3791}, DOI={10.1073/pnas.75.8.3791}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Trendelenburg, M F and Zentgraf, H and Franke, W W and Gurdon, J B}, year={1978}, month=aug, pages={3791–3795} }