DOI: 10.1073/pnas.75.12.5851. An amino-terminal fragment of <i>lac</i> repressor binds specifically to <i>lac</i> operator

An amino-terminal fragment of lac repressor binds specifically to lac operator

10.1073/pnas.75.12.5851

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have examined the effect of an aminoterminal peptide from the lac repressor (residues 1-59) on the methylation of purines in the lac operator with dimethyl sulfate. The peptide perturbs the methylation of the operator, and the peptide-induced pattern of inhibition and enhancement of methylation, across the operator, closely resembles the pattern induced by intact repressor. This demonstrates that this small amino-terminal peptide binds specifically to the lac operator and that the mechanism of recognition and binding is basically the same as that of intact repressor.

Bibliography

Ogata, R. T., & Gilbert, W. (1978). An amino-terminal fragment of lac repressor binds specifically to lac operator. Proceedings of the National Academy of Sciences, 75(12), 5851â5854.

Authors 2

Ronald T. Ogata (first)
Walter Gilbert (additional)

References 0 Referenced 87

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:38 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:10 a.m.)
Indexed	1 year, 6 months ago (Feb. 23, 2024, 12:47 p.m.)
Issued	46 years, 8 months ago (Dec. 1, 1978)
Published	46 years, 8 months ago (Dec. 1, 1978)
Published Online	46 years, 8 months ago (Dec. 1, 1978)
Published Print	46 years, 8 months ago (Dec. 1, 1978)

Funders 0

None

BibTeX

@article{Ogata_1978, title={An amino-terminal fragment of lac repressor binds specifically to lac operator}, volume={75}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.75.12.5851}, DOI={10.1073/pnas.75.12.5851}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ogata, Ronald T. and Gilbert, Walter}, year={1978}, month=dec, pages={5851–5854} }

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