Abstract
Correct assembly of the heads of bacteriophages lambda and T4 requires the function of the groE gene of the Escherichia coli host. We have isolated a transducing derivative of lambda, called lambda gt-Ec.groE, that carries a functional copy of the groE gene. Unlike wild-type lambda, this phage is able to form plaques on hosts with a mutant groE gene. We have isolated an amber mutation in the groE gene carried by the phage, and this has made it possible to identify the groE product as a protein of molecular weight 65,000. In the phage, the groE gene is under the control of an early phage promoter.
Dates
Type | When |
---|---|
Created | 19 years, 3 months ago (May 31, 2006, 3:34 a.m.) |
Deposited | 3 years, 4 months ago (April 13, 2022, 11:13 a.m.) |
Indexed | 1 year, 11 months ago (Sept. 14, 2023, 10:25 p.m.) |
Issued | 47 years, 8 months ago (Jan. 1, 1978) |
Published | 47 years, 8 months ago (Jan. 1, 1978) |
Published Online | 47 years, 8 months ago (Jan. 1, 1978) |
Published Print | 47 years, 8 months ago (Jan. 1, 1978) |
@article{Hendrix_1978, title={Role of the host in virus assembly: cloning of the Escherichia coli groE gene and identification of its protein product.}, volume={75}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.75.1.136}, DOI={10.1073/pnas.75.1.136}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hendrix, R W and Tsui, L}, year={1978}, month=jan, pages={136–139} }