DOI: 10.1073/pnas.74.9.3937. Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids.

Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids.

10.1073/pnas.74.9.3937

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Trispecific microcell hybrids were prepared by transferring limited numbers of chromosomes from a human/mouse gene-transfer cell line to a Chinese hamster recipient line. The donor cells employed were murine L-cells that stably expressed the human form of the enzyme hypoxanthine phosphoribosyltransferase. Karyotypic, zymographic, and back-selection tests of the resulting human/mouse/Chinese hamster microcell hybrids provided strong genetic evidence for a stable association of the human transgenome with host murine chromosomes in stable gene-transfer cell lines. This association, which may represent physical integration of the transgenome into the host cell genome, occurred at multiple chromosomal sites.

Bibliography

Fournier, R. E., & Ruddle, F. H. (1977). Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids. Proceedings of the National Academy of Sciences, 74(9), 3937â3941.

Authors 2

R E Fournier (first)
F H Ruddle (additional)

References 0 Referenced 37

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:34 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:55 a.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:17 a.m.)
Issued	47 years, 11 months ago (Sept. 1, 1977)
Published	47 years, 11 months ago (Sept. 1, 1977)
Published Online	47 years, 11 months ago (Sept. 1, 1977)
Published Print	47 years, 11 months ago (Sept. 1, 1977)

Funders 0

None

BibTeX

@article{Fournier_1977, title={Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids.}, volume={74}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.74.9.3937}, DOI={10.1073/pnas.74.9.3937}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Fournier, R E and Ruddle, F H}, year={1977}, month=sep, pages={3937–3941} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        11
      ]
    ],
    "date-time": "2025-07-11T10:17:08Z",
    "timestamp": 1752229028116
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "9",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1977,
        9
      ]
    ]
  },
  "abstract": "<jats:p>Trispecific microcell hybrids were prepared by transferring limited numbers of chromosomes from a human/mouse gene-transfer cell line to a Chinese hamster recipient line. The donor cells employed were murine L-cells that stably expressed the human form of the enzyme hypoxanthine phosphoribosyltransferase. Karyotypic, zymographic, and back-selection tests of the resulting human/mouse/Chinese hamster microcell hybrids provided strong genetic evidence for a stable association of the human transgenome with host murine chromosomes in stable gene-transfer cell lines. This association, which may represent physical integration of the transgenome into the host cell genome, occurred at multiple chromosomal sites.</jats:p>",
  "DOI": "10.1073/pnas.74.9.3937",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T07:34:19Z",
    "timestamp": 1149060859000
  },
  "page": "3937-3941",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 37,
  "title": "Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids.",
  "prefix": "10.1073",
  "volume": "74",
  "author": [
    {
      "given": "R E",
      "family": "Fournier",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "F H",
      "family": "Ruddle",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1977,
        9
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.74.9.3937",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T14:55:06Z",
    "timestamp": 1649861706000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.74.9.3937"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1977,
        9
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "9",
    "published-print": {
      "date-parts": [
        [
          1977,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.74.9.3937"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.74.9.3937",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1977,
        9
      ]
    ]
  },
  "assertion": [
    {
      "value": "1977-09-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}