DOI: 10.1073/pnas.74.9.3918. Variants of 3T3 cells lacking mitogenic response to epidermal growth factor.

Variants of 3T3 cells lacking mitogenic response to epidermal growth factor.

10.1073/pnas.74.9.3918

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have previously demonstrated that epidermal growth factor (EGF) can serve as a potent mitogen for 3T3 cells. We have now selected variant 3T3 cell lines unable to respond to EGF, in order to define cellular events unique to the EGF response and to distinguish which of these events are necessary and which are merely correlative to mitogenesis. By simultaneously treating cells with EGF and colchicine, we eliminated those cells stimulated by EGF to enter mitosis. Of the eight clonal EGF nonresponder variants selected by this procedure, none retains a functional EGF receptor. The EGF nonresponsive va-riant lines still retain the ability to respond to other mitogens.

Bibliography

Pruss, R. M., & Herschman, H. R. (1977). Variants of 3T3 cells lacking mitogenic response to epidermal growth factor. Proceedings of the National Academy of Sciences, 74(9), 3918â3921.

Authors 2

R M Pruss (first)
H R Herschman (additional)

References 0 Referenced 192

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:34 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:56 a.m.)
Indexed	1 year, 1 month ago (July 11, 2024, 11:56 a.m.)
Issued	48 years ago (Sept. 1, 1977)
Published	48 years ago (Sept. 1, 1977)
Published Online	48 years ago (Sept. 1, 1977)
Published Print	48 years ago (Sept. 1, 1977)

Funders 0

None

BibTeX

@article{Pruss_1977, title={Variants of 3T3 cells lacking mitogenic response to epidermal growth factor.}, volume={74}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.74.9.3918}, DOI={10.1073/pnas.74.9.3918}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Pruss, R M and Herschman, H R}, year={1977}, month=sep, pages={3918–3921} }

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