DOI: 10.1073/pnas.74.5.2064. High-fidelity transcription of 5S DNA injected into Xenopus oocytes.

High-fidelity transcription of 5S DNA injected into Xenopus oocytes.

10.1073/pnas.74.5.2064

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Purified DNA, from Xenopus erythrocytes, containing genes for 5S ribosomal RNA (58 DNA) is transcribed faithfully when it is injected into the nucleus of a Xenopus oocyte. Full length 5S RNA of the correct sequence is transcribed from the injected DNA, and the newly synthesized transcripts hybridize predominantly with the coding strand of 5S DNA. The majority of transcripts hybridize with the gene region as opposed to the spacer region of the coding strand. Accurate transcription starts within the first 3 hr and continues for up to 4 days after injection of 5S DNA. Radioactive 5S RNA is detected in as short a labeling time as 1 hr.

Bibliography

Brown, D. D., & Gurdon, J. B. (1977). High-fidelity transcription of 5S DNA injected into Xenopus oocytes. Proceedings of the National Academy of Sciences, 74(5), 2064â2068.

Authors 2

D D Brown (first)
J B Gurdon (additional)

References 0 Referenced 90

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:29 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:49 a.m.)
Indexed	4 months, 1 week ago (April 10, 2025, 10:09 a.m.)
Issued	48 years, 3 months ago (May 1, 1977)
Published	48 years, 3 months ago (May 1, 1977)
Published Online	48 years, 3 months ago (May 1, 1977)
Published Print	48 years, 3 months ago (May 1, 1977)

Funders 0

None

BibTeX

@article{Brown_1977, title={High-fidelity transcription of 5S DNA injected into Xenopus oocytes.}, volume={74}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.74.5.2064}, DOI={10.1073/pnas.74.5.2064}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Brown, D D and Gurdon, J B}, year={1977}, month=may, pages={2064–2068} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        4,
        10
      ]
    ],
    "date-time": "2025-04-10T14:09:46Z",
    "timestamp": 1744294186382
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "5",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1977,
        5
      ]
    ]
  },
  "abstract": "<jats:p>Purified DNA, from Xenopus erythrocytes, containing genes for 5S ribosomal RNA (58 DNA) is transcribed faithfully when it is injected into the nucleus of a Xenopus oocyte. Full length 5S RNA of the correct sequence is transcribed from the injected DNA, and the newly synthesized transcripts hybridize predominantly with the coding strand of 5S DNA. The majority of transcripts hybridize with the gene region as opposed to the spacer region of the coding strand. Accurate transcription starts within the first 3 hr and continues for up to 4 days after injection of 5S DNA. Radioactive 5S RNA is detected in as short a labeling time as 1 hr.</jats:p>",
  "DOI": "10.1073/pnas.74.5.2064",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T07:29:50Z",
    "timestamp": 1149060590000
  },
  "page": "2064-2068",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 90,
  "title": "High-fidelity transcription of 5S DNA injected into Xenopus oocytes.",
  "prefix": "10.1073",
  "volume": "74",
  "author": [
    {
      "given": "D D",
      "family": "Brown",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "J B",
      "family": "Gurdon",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1977,
        5
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.74.5.2064",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T14:49:51Z",
    "timestamp": 1649861391000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.74.5.2064"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1977,
        5
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          1977,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.74.5.2064"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.74.5.2064",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1977,
        5
      ]
    ]
  },
  "assertion": [
    {
      "value": "1977-05-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}