DOI: 10.1073/pnas.74.4.1614. Microinjection analysis of envelope-glycoprotein messenger activities of avian leukosis viral RNAs.

Microinjection analysis of envelope-glycoprotein messenger activities of avian leukosis viral RNAs.

10.1073/pnas.74.4.1614

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Virion RNA from the avian leukosis virus Rous-associated virus 2 (RAV-2) and poly(A)-containing RNAs from RAV-2-infected chick embryo fibroblasts were microinjected into fibroblasts transformed by the Bryan high-titer strain of Rous sarcoma virus (RSV), which is deficient in viral envelope glycoprotein. Production of infectious RSV following these injections depended upon the viral envelope-messenger activity of the injected RNA. This system constituted a sensitive and rigorous assay system for viral envelope-messenger RNA. It was found that 21S mRNA from RAV-2-infected cells expressed the highest activity, while 35S mRNA expressed comparatively little. In addition, RAV-2-virion RNA expressed little messenger activity. The rate of formation of infectious RSV following 21S mRNA injections reached a peak near 9 hr, which was followed by a rapid decline. Evidence has been obtained that a small fraction of both 35S virion RNA and 35S mRNA from virus-infected cells was encapsulated into virus particles following their injection into virus-producing cells.

Bibliography

Stacey, D. W., Allfrey, V. G., & Hanafusa, H. (1977). Microinjection analysis of envelope-glycoprotein messenger activities of avian leukosis viral RNAs. Proceedings of the National Academy of Sciences, 74(4), 1614â1618.

Authors 3

D W Stacey (first)
V G Allfrey (additional)
H Hanafusa (additional)

References 0 Referenced 55

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:29 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:12 a.m.)
Indexed	1 year, 10 months ago (Oct. 19, 2023, 11:03 a.m.)
Issued	48 years, 5 months ago (April 1, 1977)
Published	48 years, 5 months ago (April 1, 1977)
Published Online	48 years, 5 months ago (April 1, 1977)
Published Print	48 years, 5 months ago (April 1, 1977)

Funders 0

None

BibTeX

@article{Stacey_1977, title={Microinjection analysis of envelope-glycoprotein messenger activities of avian leukosis viral RNAs.}, volume={74}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.74.4.1614}, DOI={10.1073/pnas.74.4.1614}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Stacey, D W and Allfrey, V G and Hanafusa, H}, year={1977}, month=apr, pages={1614–1618} }

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