DOI: 10.1073/pnas.74.12.5652. Variable and constant parts of the immunoglobulin light chain gene of a mouse myeloma cell are 1250 nontranslated bases apart.

Variable and constant parts of the immunoglobulin light chain gene of a mouse myeloma cell are 1250 nontranslated bases apart.

10.1073/pnas.74.12.5652

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A DNA fragment carrying an immunoglobulin gene coding for both variable (V) and constant (C) regions of a mouse lambda light chain was enriched about 15-fold from a total endonuclease EcoRI digest of a plasmacytoma (HOPC 2020) DNA by preparative agarose gel electrophoresis. The DNA fraction was used for cloning of a lambda chain gene in the phage lambdagtWES vector. After screening [Benton, W. D. & Davis, R. W. (1977) Science 196, 180-182] of about 70,000 plaques, each arising from an independent transfection event, we isolated one clone (Ig 303) that contained both a Vlambda and a Clambda DNA sequence. Electron microscopy of R-loops formed between the cloned DNA and purified lambda chain mRNA (HOPC 2020) revealed that the Vlambda and Clambda DNA sequences are separated by a 1250-base DNA fragment.

Bibliography

Brack, C., & Tonegawa, S. (1977). Variable and constant parts of the immunoglobulin light chain gene of a mouse myeloma cell are 1250 nontranslated bases apart. Proceedings of the National Academy of Sciences, 74(12), 5652â5656.

Authors 2

C Brack (first)
S Tonegawa (additional)

References 0 Referenced 108

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:25 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:15 a.m.)
Indexed	1 day ago (Aug. 24, 2025, 7:05 p.m.)
Issued	47 years, 8 months ago (Dec. 1, 1977)
Published	47 years, 8 months ago (Dec. 1, 1977)
Published Online	47 years, 8 months ago (Dec. 1, 1977)
Published Print	47 years, 8 months ago (Dec. 1, 1977)

Funders 0

None

BibTeX

@article{Brack_1977, title={Variable and constant parts of the immunoglobulin light chain gene of a mouse myeloma cell are 1250 nontranslated bases apart.}, volume={74}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.74.12.5652}, DOI={10.1073/pnas.74.12.5652}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Brack, C and Tonegawa, S}, year={1977}, month=dec, pages={5652–5656} }

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