DOI: 10.1073/pnas.74.12.5463. DNA sequencing with chain-terminating inhibitors

DNA sequencing with chain-terminating inhibitors

10.1073/pnas.74.12.5463

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.

Authors 3

F. Sanger (first)
S. Nicklen (additional)
A. R. Coulson (additional)

References 0 Referenced 41,593

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:25 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:17 a.m.)
Indexed	1 hour, 38 minutes ago (Aug. 22, 2025, 12:47 a.m.)
Issued	47 years, 8 months ago (Dec. 1, 1977)
Published	47 years, 8 months ago (Dec. 1, 1977)
Published Online	47 years, 8 months ago (Dec. 1, 1977)
Published Print	47 years, 8 months ago (Dec. 1, 1977)

Funders 0

None

BibTeX

@article{Sanger_1977, title={DNA sequencing with chain-terminating inhibitors}, volume={74}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.74.12.5463}, DOI={10.1073/pnas.74.12.5463}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sanger, F. and Nicklen, S. and Coulson, A. R.}, year={1977}, month=dec, pages={5463–5467} }

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