DOI: 10.1073/pnas.74.11.4806. 2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.

2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.

10.1073/pnas.74.11.4806

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We measured the in vivo incorporation of 2-aminopurine into DNA of T4 bacteriophage allelic for gene 43 (DNA polymerase), mutator (L56), 43+, and antimutator (L141). The magnitude of incorporation (mol/mol of Thy) was 1/1500 in L56, 1/1600 in 43+, and 1/8900 in L141. The incorporation ratio L56:43+:L141 in vivo was equal to that mediated by the purified DNA polymerases of these allelic phages in vitro. A model for 2-aminopurine-induced A-T in equilibrium G-C transitions is discussed. The model is used to predict the magnitudes of replication errors (C mispairing with a template 2-aminopurine) and incorporation errors (2-aminopurine mispairing with a template C) per round of replication and to investigate the asymmetry in 2-aminopurine-induced transitions favoring the A-T leads to G-C pathway over G-C leads to A-T. We suggest that the fidelity of L56 and L141 DNA polymerases exemplifies one-step and two-step editing, respectively.

Bibliography

Goodman, M. F., Hopkins, R., & Gore, W. C. (1977). 2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA. Proceedings of the National Academy of Sciences, 74(11), 4806â4810.

Authors 3

M F Goodman (first)
R Hopkins (additional)
W C Gore (additional)

References 0 Referenced 37

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:24 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:11 a.m.)
Indexed	1 year, 6 months ago (Feb. 23, 2024, 12:47 p.m.)
Issued	47 years, 9 months ago (Nov. 1, 1977)
Published	47 years, 9 months ago (Nov. 1, 1977)
Published Online	47 years, 9 months ago (Nov. 1, 1977)
Published Print	47 years, 9 months ago (Nov. 1, 1977)

Funders 0

None

BibTeX

@article{Goodman_1977, title={2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.}, volume={74}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.74.11.4806}, DOI={10.1073/pnas.74.11.4806}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Goodman, M F and Hopkins, R and Gore, W C}, year={1977}, month=nov, pages={4806–4810} }

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