DOI: 10.1073/pnas.74.1.96. Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.

Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.

10.1073/pnas.74.1.96

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic methods. The starting primer, d(pT-T-A-G1, was chemically synthesized by the phosphodiester method and was extended stepwise, by reactions catalyzed by polynucleotide phosphorylase.

Bibliography

Gillam, S., Rottman, F., Jahnke, P., & Smith, M. (1977). Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene. Proceedings of the National Academy of Sciences, 74(1), 96â100.

Authors 4

S Gillam (first)
F Rottman (additional)
P Jahnke (additional)
M Smith (additional)

References 0 Referenced 24

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:21 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:07 a.m.)
Indexed	1 year ago (Aug. 27, 2024, 12:37 a.m.)
Issued	48 years, 8 months ago (Jan. 1, 1977)
Published	48 years, 8 months ago (Jan. 1, 1977)
Published Online	48 years, 8 months ago (Jan. 1, 1977)
Published Print	48 years, 8 months ago (Jan. 1, 1977)

Funders 0

None

BibTeX

@article{Gillam_1977, title={Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.}, volume={74}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.74.1.96}, DOI={10.1073/pnas.74.1.96}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gillam, S and Rottman, F and Jahnke, P and Smith, M}, year={1977}, month=jan, pages={96–100} }

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