DOI: 10.1073/pnas.73.5.1559. A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.

A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.

10.1073/pnas.73.5.1559

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Ribosomal salt washes of Artemia salina embryos contain a protein(s) that binds [3H]m7GpppGpC and [3H]m7GpppGmpC, as measured by retention on nitrocellulose membrane filters. These oligonucleotides correspond in structure to the methylated 5'-terminal sequences (caps) present in many eukaryotic mRNAs. The cap binding protein does not bind the unmethylated counterparts of caps, e.g., [32P]GpppGpCp, or a derivative of m7GpppGmpC containing ring-opened m7G. None of the purified initiation factors IF-MP, IF-M2A, IF-M2B, IF-M3, or IF-MI binds the m7G-containing oligonucleotides.

Bibliography

Filipowicz, W., Furuichi, Y., Sierra, J. M., Muthukrishnan, S., Shatkin, A. J., & Ochoa, S. (1976). A protein binding the methylated 5â-terminal sequence, m7GpppN, of eukaryotic messenger RNA. Proceedings of the National Academy of Sciences, 73(5), 1559â1563.

Authors 6

W Filipowicz (first)
Y Furuichi (additional)
J M Sierra (additional)
S Muthukrishnan (additional)
A J Shatkin (additional)
S Ochoa (additional)

References 0 Referenced 89

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:17 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:57 a.m.)
Indexed	1 month ago (Aug. 2, 2025, 12:28 a.m.)
Issued	49 years, 4 months ago (May 1, 1976)
Published	49 years, 4 months ago (May 1, 1976)
Published Online	49 years, 4 months ago (May 1, 1976)
Published Print	49 years, 4 months ago (May 1, 1976)

Funders 0

None

BibTeX

@article{Filipowicz_1976, title={A protein binding the methylated 5’-terminal sequence, m7GpppN, of eukaryotic messenger RNA.}, volume={73}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.73.5.1559}, DOI={10.1073/pnas.73.5.1559}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Filipowicz, W and Furuichi, Y and Sierra, J M and Muthukrishnan, S and Shatkin, A J and Ochoa, S}, year={1976}, month=may, pages={1559–1563} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        2
      ]
    ],
    "date-time": "2025-08-02T04:28:23Z",
    "timestamp": 1754108903941
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "5",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1976,
        5
      ]
    ]
  },
  "abstract": "<jats:p>Ribosomal salt washes of Artemia salina embryos contain a protein(s) that binds [3H]m7GpppGpC and [3H]m7GpppGmpC, as measured by retention on nitrocellulose membrane filters. These oligonucleotides correspond in structure to the methylated 5'-terminal sequences (caps) present in many eukaryotic mRNAs. The cap binding protein does not bind the unmethylated counterparts of caps, e.g., [32P]GpppGpCp, or a derivative of m7GpppGmpC containing ring-opened m7G. None of the purified initiation factors IF-MP, IF-M2A, IF-M2B, IF-M3, or IF-MI binds the m7G-containing oligonucleotides.</jats:p>",
  "DOI": "10.1073/pnas.73.5.1559",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T07:17:38Z",
    "timestamp": 1149059858000
  },
  "page": "1559-1563",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 89,
  "title": "A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.",
  "prefix": "10.1073",
  "volume": "73",
  "author": [
    {
      "given": "W",
      "family": "Filipowicz",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Y",
      "family": "Furuichi",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J M",
      "family": "Sierra",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "S",
      "family": "Muthukrishnan",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "A J",
      "family": "Shatkin",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "S",
      "family": "Ochoa",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1976,
        5
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.73.5.1559",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T14:57:32Z",
    "timestamp": 1649861852000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.73.5.1559"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1976,
        5
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          1976,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.73.5.1559"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.73.5.1559",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1976,
        5
      ]
    ]
  },
  "assertion": [
    {
      "value": "1976-05-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}