DOI: 10.1073/pnas.73.12.4474. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.

Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.

10.1073/pnas.73.12.4474

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Novobiocin and coumermycin are known to inhibit the replication of DNA iing of DNA catalyzed by E. coli DNA gyrase, a recently discovered enzyme that introduces negative superhelical turns into covalently circular DNA. The activity of DNA gyrase purified from a coumermycin-resistant mutant strain is resistant to both drugs. The inhibition by novobiocin of colicin E1 plasmid DNA replication in a cell-free system is partially relieved by adding resistant DNA gyrase. Both in the case of coliclls. DNA molecules which are converted to the covalently circular form in thepresence of coumermycin remain relaxed, instead of achieving their normal supercoiled conformation. We conclude that DNA gyrase controls the supercoiling of DNA in E. coli.

Bibliography

Gellert, M., OâDea, M. H., Itoh, T., & Tomizawa, J. (1976). Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proceedings of the National Academy of Sciences, 73(12), 4474â4478.

Authors 4

M Gellert (first)
M H O'Dea (additional)
T Itoh (additional)
J Tomizawa (additional)

References 0 Referenced 529

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:13 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:59 a.m.)
Indexed	1 month, 1 week ago (July 19, 2025, 11:29 p.m.)
Issued	48 years, 9 months ago (Dec. 1, 1976)
Published	48 years, 9 months ago (Dec. 1, 1976)
Published Online	48 years, 9 months ago (Dec. 1, 1976)
Published Print	48 years, 9 months ago (Dec. 1, 1976)

Funders 0

None

BibTeX

@article{Gellert_1976, title={Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.}, volume={73}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.73.12.4474}, DOI={10.1073/pnas.73.12.4474}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gellert, M and O’Dea, M H and Itoh, T and Tomizawa, J}, year={1976}, month=dec, pages={4474–4478} }

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