DOI: 10.1073/pnas.72.9.3416. In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase.

In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase.

10.1073/pnas.72.9.3416

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

DNA from lambdagt-lambdaB bacteriophage was cleaved with EcoRI endonuclease and fragments from EcoRI-digested E. coli DNA were inserted. This DNA was used to infect E. coli, and phages containing the gene for DNA ligase were isolated by genetic selection. Two different hybrids were found with the same E. coli segment inserted in opposite orientations. Both hybrids produced similar levels of ligase as measured in crude extracts of infected cells.

Bibliography

Cameron, J. R., Panasenko, S. M., Lehman, I. R., & Davis, R. W. (1975). In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase. Proceedings of the National Academy of Sciences, 72(9), 3416â3420.

Authors 4

J R Cameron (first)
S M Panasenko (additional)
I R Lehman (additional)
R W Davis (additional)

References 0 Referenced 62

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:10 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:07 a.m.)
Indexed	1 year, 7 months ago (Jan. 14, 2024, 11:58 a.m.)
Issued	49 years, 11 months ago (Sept. 1, 1975)
Published	49 years, 11 months ago (Sept. 1, 1975)
Published Online	49 years, 11 months ago (Sept. 1, 1975)
Published Print	49 years, 11 months ago (Sept. 1, 1975)

Funders 0

None

BibTeX

@article{Cameron_1975, title={In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase.}, volume={72}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.72.9.3416}, DOI={10.1073/pnas.72.9.3416}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Cameron, J R and Panasenko, S M and Lehman, I R and Davis, R W}, year={1975}, month=sep, pages={3416–3420} }

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