DOI: 10.1073/pnas.72.7.2626. An octamer of histones in chromatin and free in solution.

An octamer of histones in chromatin and free in solution.

10.1073/pnas.72.7.2626

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Crosslinking with dimethyl suberimidate reveals a chain of histone octamers in chromatin. The octamer can be isolated free in solution at high ionic strength and pH. The identification of dimers formed by crosslinking reveals two or more contacts of each histone with others within the octamer. The molecular weight (110,000) and pattern of dissociation of the octamer are compatible with the composition (F2A1)2(F3)2(F2A2)2(F2B)2.

Bibliography

Thomas, J. O., & Kornberg, R. D. (1975). An octamer of histones in chromatin and free in solution. Proceedings of the National Academy of Sciences, 72(7), 2626â2630.

Authors 2

J O Thomas (first)
R D Kornberg (additional)

References 0 Referenced 419

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:09 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:04 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 6, 2025, 9:04 a.m.)
Issued	50 years, 1 month ago (July 1, 1975)
Published	50 years, 1 month ago (July 1, 1975)
Published Online	50 years, 1 month ago (July 1, 1975)
Published Print	50 years, 1 month ago (July 1, 1975)

Funders 0

None

BibTeX

@article{Thomas_1975, title={An octamer of histones in chromatin and free in solution.}, volume={72}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.72.7.2626}, DOI={10.1073/pnas.72.7.2626}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Thomas, J O and Kornberg, R D}, year={1975}, month=jul, pages={2626–2630} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        6
      ]
    ],
    "date-time": "2025-08-06T13:04:34Z",
    "timestamp": 1754485474948
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "7",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1975,
        7
      ]
    ]
  },
  "abstract": "<jats:p>Crosslinking with dimethyl suberimidate reveals a chain of histone octamers in chromatin. The octamer can be isolated free in solution at high ionic strength and pH. The identification of dimers formed by crosslinking reveals two or more contacts of each histone with others within the octamer. The molecular weight (110,000) and pattern of dissociation of the octamer are compatible with the composition (F2A1)2(F3)2(F2A2)2(F2B)2.</jats:p>",
  "DOI": "10.1073/pnas.72.7.2626",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T07:09:03Z",
    "timestamp": 1149059343000
  },
  "page": "2626-2630",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 419,
  "title": "An octamer of histones in chromatin and free in solution.",
  "prefix": "10.1073",
  "volume": "72",
  "author": [
    {
      "given": "J O",
      "family": "Thomas",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "R D",
      "family": "Kornberg",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1975,
        7
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.72.7.2626",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T15:04:19Z",
    "timestamp": 1649862259000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.72.7.2626"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1975,
        7
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "7",
    "published-print": {
      "date-parts": [
        [
          1975,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.72.7.2626"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.72.7.2626",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1975,
        7
      ]
    ]
  },
  "assertion": [
    {
      "value": "1975-07-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}