DOI: 10.1073/pnas.72.4.1594. Immunological and chemical purity of papain-solubilized HL-A antigens.

Immunological and chemical purity of papain-solubilized HL-A antigens.

10.1073/pnas.72.4.1594

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Three preparations of purified papain-solublized HL-A antigens have been radiolabeled by reductive methylation using formaldehyde and potassium boro[3H]hydride, and their reaction with specific HL-A antisera has been investigated. Greater than 99 percent of the radioactivity in the [3H]HL-A2 preparation could be complexed with several HL-A2 antisera, but not with specificity controls. The other two preparations, which contained mixtures of HL-A antigenic specificities (HL-A7,12 an HL-A3,W25;12,27), showed 63 per cent and 70 per cent complex formation with mixtures of the appropriate HL-A antisera. The N-terminal amino acid of both subunits has been determined for the three HL-A antigen preparations. In all cases the only detectable N-terminal amino acids were isoleucine for the small subunits, beta-2-microblogulin, and glycine for the larger subunit.

Bibliography

Parham, P., Terhorst, C., Herrmann, H., Humphreys, R. E., Waterfield, M. D., & Strominger, J. L. (1975). Immunological and chemical purity of papain-solubilized HL-A antigens. Proceedings of the National Academy of Sciences, 72(4), 1594â1598.

Authors 6

P Parham (first)
C Terhorst (additional)
H Herrmann (additional)
R E Humphreys (additional)
M D Waterfield (additional)
J L Strominger (additional)

References 0 Referenced 17

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:05 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:03 a.m.)
Indexed	1 year, 9 months ago (Nov. 10, 2023, 7:36 p.m.)
Issued	50 years, 5 months ago (April 1, 1975)
Published	50 years, 5 months ago (April 1, 1975)
Published Online	50 years, 5 months ago (April 1, 1975)
Published Print	50 years, 5 months ago (April 1, 1975)

Funders 0

None

BibTeX

@article{Parham_1975, title={Immunological and chemical purity of papain-solubilized HL-A antigens.}, volume={72}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.72.4.1594}, DOI={10.1073/pnas.72.4.1594}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Parham, P and Terhorst, C and Herrmann, H and Humphreys, R E and Waterfield, M D and Strominger, J L}, year={1975}, month=apr, pages={1594–1598} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        11
      ]
    ],
    "date-time": "2023-11-11T00:36:58Z",
    "timestamp": 1699663018920
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "4",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1975,
        4
      ]
    ]
  },
  "abstract": "<jats:p>Three preparations of purified papain-solublized HL-A antigens have been radiolabeled by reductive methylation using formaldehyde and potassium boro[3H]hydride, and their reaction with specific HL-A antisera has been investigated. Greater than 99 percent of the radioactivity in the [3H]HL-A2 preparation could be complexed with several HL-A2 antisera, but not with specificity controls. The other two preparations, which contained mixtures of HL-A antigenic specificities (HL-A7,12 an HL-A3,W25;12,27), showed 63 per cent and 70 per cent complex formation with mixtures of the appropriate HL-A antisera. The N-terminal amino acid of both subunits has been determined for the three HL-A antigen preparations. In all cases the only detectable N-terminal amino acids were isoleucine for the small subunits, beta-2-microblogulin, and glycine for the larger subunit.</jats:p>",
  "DOI": "10.1073/pnas.72.4.1594",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T07:05:56Z",
    "timestamp": 1149059156000
  },
  "page": "1594-1598",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 17,
  "title": "Immunological and chemical purity of papain-solubilized HL-A antigens.",
  "prefix": "10.1073",
  "volume": "72",
  "author": [
    {
      "given": "P",
      "family": "Parham",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "C",
      "family": "Terhorst",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "H",
      "family": "Herrmann",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "R E",
      "family": "Humphreys",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M D",
      "family": "Waterfield",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J L",
      "family": "Strominger",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1975,
        4
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.72.4.1594",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T15:03:33Z",
    "timestamp": 1649862213000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.72.4.1594"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1975,
        4
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1975,
          4
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.72.4.1594"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.72.4.1594",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1975,
        4
      ]
    ]
  },
  "assertion": [
    {
      "value": "1975-04-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}