Abstract
Chromatin prepared by gentle methods from mouse myeloma cells retained its ability to synthesize RNA using bound endogenous RNA polymerase (RNA nucleotidyltransferase; nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6). The transcription resembles that observed in vivo in several respects. The low-molecular-weight RNA species 5S RNA and the 4.5S precursor to 4S RNA, are transcribed accurately and transcription is reinitiated continually in vitro. Their synthesis was not inhibited by alpha-amanitin (1 mug/ml) as was found previously for these species in isolated nuclei.
Dates
Type | When |
---|---|
Created | 19 years, 3 months ago (May 31, 2006, 3:05 a.m.) |
Deposited | 3 years, 4 months ago (April 13, 2022, 10:33 a.m.) |
Indexed | 1 year, 9 months ago (Nov. 23, 2023, 2:07 a.m.) |
Issued | 50 years, 6 months ago (March 1, 1975) |
Published | 50 years, 6 months ago (March 1, 1975) |
Published Online | 50 years, 6 months ago (March 1, 1975) |
Published Print | 50 years, 6 months ago (March 1, 1975) |
@article{Marzluff_1975, title={Chromatin directed transcription of 5S and tRNA genes.}, volume={72}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.72.3.1082}, DOI={10.1073/pnas.72.3.1082}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Marzluff, W F and Huang, R C}, year={1975}, month=mar, pages={1082–1086} }