Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Chromatin prepared by gentle methods from mouse myeloma cells retained its ability to synthesize RNA using bound endogenous RNA polymerase (RNA nucleotidyltransferase; nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6). The transcription resembles that observed in vivo in several respects. The low-molecular-weight RNA species 5S RNA and the 4.5S precursor to 4S RNA, are transcribed accurately and transcription is reinitiated continually in vitro. Their synthesis was not inhibited by alpha-amanitin (1 mug/ml) as was found previously for these species in isolated nuclei.

Bibliography

Marzluff, W. F., & Huang, R. C. (1975). Chromatin directed transcription of 5S and tRNA genes. Proceedings of the National Academy of Sciences, 72(3), 1082–1086.

Authors 2
  1. W F Marzluff (first)
  2. R C Huang (additional)
References 0 Referenced 29

None

Dates
Type When
Created 19 years, 3 months ago (May 31, 2006, 3:05 a.m.)
Deposited 3 years, 4 months ago (April 13, 2022, 10:33 a.m.)
Indexed 1 year, 9 months ago (Nov. 23, 2023, 2:07 a.m.)
Issued 50 years, 6 months ago (March 1, 1975)
Published 50 years, 6 months ago (March 1, 1975)
Published Online 50 years, 6 months ago (March 1, 1975)
Published Print 50 years, 6 months ago (March 1, 1975)
Funders 0

None

@article{Marzluff_1975, title={Chromatin directed transcription of 5S and tRNA genes.}, volume={72}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.72.3.1082}, DOI={10.1073/pnas.72.3.1082}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Marzluff, W F and Huang, R C}, year={1975}, month=mar, pages={1082–1086} }