DOI: 10.1073/pnas.72.3.1077. Possible relationship of poly(A) shortening to mRNA turnover.

Possible relationship of poly(A) shortening to mRNA turnover.

10.1073/pnas.72.3.1077

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Whereas the original size of poly(A) in HeLa cells is about 200 nucleotides, at steady state most of the poly(A) in mRNA contains less than 50 nucleotides. An endonucleolytic attack on poly(A) is suggested as the most likely method to accumulate short pieces of poly(A). Both poly(A) shortening and mRNA turnover appear to be inhibited by emetine, a drug that stops translation. It is possible that a random endonucleolytic attack leads to scission of poly(A) to a size below which the mRNA is unstable.

Bibliography

Sheiness, D., Puckett, L., & Darnell, J. E. (1975). Possible relationship of poly(A) shortening to mRNA turnover. Proceedings of the National Academy of Sciences, 72(3), 1077â1081.

Authors 3

D Sheiness (first)
L Puckett (additional)
J E Darnell (additional)

References 0 Referenced 67

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:05 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:33 a.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 11:53 a.m.)
Issued	50 years, 6 months ago (March 1, 1975)
Published	50 years, 6 months ago (March 1, 1975)
Published Online	50 years, 6 months ago (March 1, 1975)
Published Print	50 years, 6 months ago (March 1, 1975)

Funders 0

None

BibTeX

@article{Sheiness_1975, title={Possible relationship of poly(A) shortening to mRNA turnover.}, volume={72}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.72.3.1077}, DOI={10.1073/pnas.72.3.1077}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sheiness, D and Puckett, L and Darnell, J E}, year={1975}, month=mar, pages={1077–1081} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        10
      ]
    ],
    "date-time": "2024-07-10T15:53:52Z",
    "timestamp": 1720626832368
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "3",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1975,
        3
      ]
    ]
  },
  "abstract": "<jats:p>Whereas the original size of poly(A) in HeLa cells is about 200 nucleotides, at steady state most of the poly(A) in mRNA contains less than 50 nucleotides. An endonucleolytic attack on poly(A) is suggested as the most likely method to accumulate short pieces of poly(A). Both poly(A) shortening and mRNA turnover appear to be inhibited by emetine, a drug that stops translation. It is possible that a random endonucleolytic attack leads to scission of poly(A) to a size below which the mRNA is unstable.</jats:p>",
  "DOI": "10.1073/pnas.72.3.1077",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T07:05:44Z",
    "timestamp": 1149059144000
  },
  "page": "1077-1081",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 67,
  "title": "Possible relationship of poly(A) shortening to mRNA turnover.",
  "prefix": "10.1073",
  "volume": "72",
  "author": [
    {
      "given": "D",
      "family": "Sheiness",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "L",
      "family": "Puckett",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J E",
      "family": "Darnell",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1975,
        3
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.72.3.1077",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T14:33:14Z",
    "timestamp": 1649860394000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.72.3.1077"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1975,
        3
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1975,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.72.3.1077"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.72.3.1077",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1975,
        3
      ]
    ]
  },
  "assertion": [
    {
      "value": "1975-03-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}