DOI: 10.1073/pnas.72.2.742. 5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.

5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.

10.1073/pnas.72.2.742

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Methylated reovirus mRNA was synthesized in vitro in the presence of S-adenosyl-L-[methyl-3H]-methionine. Viral genome double-stranded RNA that was uniformly labeled with 32-P was isolated from purified virions. The RNAs were mixed and their 5'-terminal structures compared by electrophoretic and chromatographic analyses after enzymatic digestion. Both the mRNA and the corresponding strand in the genome RNA contain m-7G(5')ppp(5')G-m-pCp, indicating that infected cells synthesize viral RNA with blocked, methylated 5' termini.

Bibliography

Furuichi, Y., Muthukrishnan, S., & Shatkin, A. J. (1975). 5â-Terminal m-7G(5â)ppp(5â)G-m-p in vivo: identification in reovirus genome RNA. Proceedings of the National Academy of Sciences, 72(2), 742â745.

Authors 3

Y Furuichi (first)
S Muthukrishnan (additional)
A J Shatkin (additional)

References 0 Referenced 99

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:04 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:23 a.m.)
Indexed	4 months, 3 weeks ago (April 10, 2025, 6:56 a.m.)
Issued	50 years, 7 months ago (Feb. 1, 1975)
Published	50 years, 7 months ago (Feb. 1, 1975)
Published Online	50 years, 7 months ago (Feb. 1, 1975)
Published Print	50 years, 7 months ago (Feb. 1, 1975)

Funders 0

None

BibTeX

@article{Furuichi_1975, title={5’-Terminal m-7G(5’)ppp(5’)G-m-p in vivo: identification in reovirus genome RNA.}, volume={72}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.72.2.742}, DOI={10.1073/pnas.72.2.742}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Furuichi, Y and Muthukrishnan, S and Shatkin, A J}, year={1975}, month=feb, pages={742–745} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        4,
        10
      ]
    ],
    "date-time": "2025-04-10T10:56:51Z",
    "timestamp": 1744282611212
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "2",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1975,
        2
      ]
    ]
  },
  "abstract": "<jats:p>Methylated reovirus mRNA was synthesized in vitro in the presence of S-adenosyl-L-[methyl-3H]-methionine. Viral genome double-stranded RNA that was uniformly labeled with 32-P was isolated from purified virions. The RNAs were mixed and their 5'-terminal structures compared by electrophoretic and chromatographic analyses after enzymatic digestion. Both the mRNA and the corresponding strand in the genome RNA contain m-7G(5')ppp(5')G-m-pCp, indicating that infected cells synthesize viral RNA with blocked, methylated 5' termini.</jats:p>",
  "DOI": "10.1073/pnas.72.2.742",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T07:04:50Z",
    "timestamp": 1149059090000
  },
  "page": "742-745",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 99,
  "title": "5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.",
  "prefix": "10.1073",
  "volume": "72",
  "author": [
    {
      "given": "Y",
      "family": "Furuichi",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "S",
      "family": "Muthukrishnan",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "A J",
      "family": "Shatkin",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1975,
        2
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.72.2.742",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T14:23:31Z",
    "timestamp": 1649859811000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.72.2.742"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1975,
        2
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1975,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.72.2.742"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.72.2.742",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1975,
        2
      ]
    ]
  },
  "assertion": [
    {
      "value": "1975-02-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}