DOI: 10.1073/pnas.71.9.3492. Isolation of a Major Cell Surface Glycoprotein from Fibroblasts

Isolation of a Major Cell Surface Glycoprotein from Fibroblasts

10.1073/pnas.71.9.3492

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A cell surface component has been isolated in partially purified form from cultured chick embryo and chick heart fibroblasts. This glycoprotein is similar to a protein recently reported to be present at the surface of normal cells, and missing after neoplastic transformation. It is a major cell surface glycoprotein that is synthesized by cultured fibroblasts, but is not collagen. It is shown to be markedly trypsin-sensitive, and its recovery from the cell surface is dependent on cell density. It is excluded from Sephadex G-200, but is not rapidly sedimented by ultracentrifugation, and has an apparent molecular weight of 220,000. The isolation of this cell surface glycoprotein may now provide a means of determining its function.

Bibliography

Yamada, K. M., & Weston, J. A. (1974). Isolation of a Major Cell Surface Glycoprotein from Fibroblasts. Proceedings of the National Academy of Sciences, 71(9), 3492â3496.

Authors 2

Kenneth M. Yamada (first)
James A. Weston (additional)

References 0 Referenced 169

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:59 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:49 a.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 7:12 a.m.)
Issued	51 years ago (Sept. 1, 1974)
Published	51 years ago (Sept. 1, 1974)
Published Online	51 years ago (Sept. 1, 1974)
Published Print	51 years ago (Sept. 1, 1974)

Funders 0

None

BibTeX

@article{Yamada_1974, title={Isolation of a Major Cell Surface Glycoprotein from Fibroblasts}, volume={71}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.71.9.3492}, DOI={10.1073/pnas.71.9.3492}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yamada, Kenneth M. and Weston, James A.}, year={1974}, month=sep, pages={3492–3496} }

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