DOI: 10.1073/pnas.71.8.3172. Genetic Recombination and Commitment to Meiosis in <i>Saccharomyces</i>

Genetic Recombination and Commitment to Meiosis in Saccharomyces

10.1073/pnas.71.8.3172

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Diploid cells of the yeast Saccharomyces cerevisiae become committed to recombination at meiotic levels without becoming committed to the meiotic disjunction of chromosomes. These two events of the meiotic process can be separated by removing cells from a meiosis-inducing medium and returning them to a medium that promotes vegetative cell division. Cells removed at an appropriate time remain diploid, revert to mitosis, and display recombination with meiotic-like frequencies. Those removed after this time are committed to the completion of meiosis. Diploids of three conditional sporulation-deficient mutants ( spo 1-1, spo 2-1, and spo 3-1) have been examined for recombination at restrictive temperatures. All exhibit commitment to recombination without commitment to meiotic disjunction as in the wild type. Cells of spo 1-1/ spo 1-1 do not replicate the spindle pole body for meiosis I; thus, recombination ability can be acquired by cells that do not proceed beyond this cytological stage.

Bibliography

Esposito, R. E., & Esposito, M. S. (1974). Genetic Recombination and Commitment to Meiosis in Saccharomyces. Proceedings of the National Academy of Sciences, 71(8), 3172â3176.

Authors 2

Rochelle E. Esposito (first)
Michael S. Esposito (additional)

References 0 Referenced 95

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:59 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:22 a.m.)
Indexed	3 days, 14 hours ago (Aug. 26, 2025, 2:31 a.m.)
Issued	51 years ago (Aug. 1, 1974)
Published	51 years ago (Aug. 1, 1974)
Published Online	51 years ago (Aug. 1, 1974)
Published Print	51 years ago (Aug. 1, 1974)

Funders 0

None

BibTeX

@article{Esposito_1974, title={Genetic Recombination and Commitment to Meiosis in Saccharomyces}, volume={71}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.71.8.3172}, DOI={10.1073/pnas.71.8.3172}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Esposito, Rochelle E. and Esposito, Michael S.}, year={1974}, month=aug, pages={3172–3176} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        26
      ]
    ],
    "date-time": "2025-08-26T06:31:27Z",
    "timestamp": 1756189887497
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "8",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1974,
        8
      ]
    ]
  },
  "abstract": "<jats:p>\n            Diploid cells of the yeast\n            <jats:italic>Saccharomyces cerevisiae</jats:italic>\n            become committed to recombination at meiotic levels without becoming committed to the meiotic disjunction of chromosomes. These two events of the meiotic process can be separated by removing cells from a meiosis-inducing medium and returning them to a medium that promotes vegetative cell division. Cells removed at an appropriate time remain diploid, revert to mitosis, and display recombination with meiotic-like frequencies. Those removed after this time are committed to the completion of meiosis. Diploids of three conditional sporulation-deficient mutants (\n            <jats:italic>spo</jats:italic>\n            1-1,\n            <jats:italic>spo</jats:italic>\n            2-1, and\n            <jats:italic>spo</jats:italic>\n            3-1) have been examined for recombination at restrictive temperatures. All exhibit commitment to recombination without commitment to meiotic disjunction as in the wild type. Cells of\n            <jats:italic>spo</jats:italic>\n            1-1/\n            <jats:italic>spo</jats:italic>\n            1-1 do not replicate the spindle pole body for meiosis I; thus, recombination ability can be acquired by cells that do not proceed beyond this cytological stage.\n          </jats:p>",
  "DOI": "10.1073/pnas.71.8.3172",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T06:59:12Z",
    "timestamp": 1149058752000
  },
  "page": "3172-3176",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 95,
  "title": "Genetic Recombination and Commitment to Meiosis in\n            <i>Saccharomyces</i>",
  "prefix": "10.1073",
  "volume": "71",
  "author": [
    {
      "given": "Rochelle E.",
      "family": "Esposito",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Erman Biology Center, Department of Biology, University of Chicago, Chicago, Illinois 60637"
        }
      ]
    },
    {
      "given": "Michael S.",
      "family": "Esposito",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Erman Biology Center, Department of Biology, University of Chicago, Chicago, Illinois 60637"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1974,
        8
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.71.8.3172",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T14:22:52Z",
    "timestamp": 1649859772000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.71.8.3172"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1974,
        8
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "8",
    "published-print": {
      "date-parts": [
        [
          1974,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.71.8.3172"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.71.8.3172",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1974,
        8
      ]
    ]
  },
  "assertion": [
    {
      "value": "1974-08-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}