Abstract
A precursor head of phage lambda is synthesized after induction of cells lysogenic for λ D - F - and λ A - (head-defective) mutants. This precursor head can be assayed by complementation in vitro and can be purified by CsCl gradient centrifugation and sucrose gradient sedimentation. The precursor head contains no DNA and has the same dimensions as the petit λ particle. It can be packed with λ DNA in an extract from induced Escherichia coli lysogenic for a λ E - mutant.
Dates
Type | When |
---|---|
Created | 19 years, 3 months ago (May 31, 2006, 2:56 a.m.) |
Deposited | 3 years, 4 months ago (April 13, 2022, 10:27 a.m.) |
Indexed | 1 year, 7 months ago (Jan. 15, 2024, 5:43 a.m.) |
Issued | 51 years, 3 months ago (June 1, 1974) |
Published | 51 years, 3 months ago (June 1, 1974) |
Published Online | 51 years, 3 months ago (June 1, 1974) |
Published Print | 51 years, 3 months ago (June 1, 1974) |
@article{Hohn_1974, title={Activity of Empty, Headlike Particles for Packaging of DNA of Bacteriophage λ In Vitro}, volume={71}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.71.6.2372}, DOI={10.1073/pnas.71.6.2372}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hohn, B. and Hohn, T.}, year={1974}, month=jun, pages={2372–2376} }