DOI: 10.1073/pnas.71.5.1590. Regulation of Melanocyte Stimulating Hormone Action at the Receptor Level: Discontinuous Binding of Hormone to Synchronized Mouse Melanoma Cells During the Cell Cycle

Regulation of Melanocyte Stimulating Hormone Action at the Receptor Level: Discontinuous Binding of Hormone to Synchronized Mouse Melanoma Cells During the Cell Cycle

10.1073/pnas.71.5.1590

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Melanocyte stimulating hormone coupled to Sepharose effects an increase in tryosinase (EC 1.14.18.1; monophenol monoxygenase) activity of cultivated mouse melanoma cells. Synchronized cells are found to respond to melanocyte stimulating hormone only in the G2 phase of the cell cycle, although their response to cyclic AMP is independent of position in the cell cycle. The binding of 125 I-labeled melanocyte stimulating hormone occurs predominantly in G2. These observations are satisfied by a model in which the hormone can activate adenylate cyclase (EC 4.6.1.1) by binding to a melanocyte stimulating hormone receptor only in G2; the events distal to cyclic AMP production can occur throughout the cell cycle.

Bibliography

Varga, J. M., Dipasquale, A., Pawelek, J., McGuire, J. S., & Lerner, A. B. (1974). Regulation of Melanocyte Stimulating Hormone Action at the Receptor Level: Discontinuous Binding of Hormone to Synchronized Mouse Melanoma Cells During the Cell Cycle. Proceedings of the National Academy of Sciences, 71(5), 1590â1593.

Authors 5

J. M. Varga (first)
A. Dipasquale (additional)
J. Pawelek (additional)
J. S. McGuire (additional)
A. B. Lerner (additional)

References 0 Referenced 81

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:55 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:26 a.m.)
Indexed	1 month ago (Aug. 2, 2025, 12:17 a.m.)
Issued	51 years, 4 months ago (May 1, 1974)
Published	51 years, 4 months ago (May 1, 1974)
Published Online	51 years, 4 months ago (May 1, 1974)
Published Print	51 years, 4 months ago (May 1, 1974)

Funders 0

None

BibTeX

@article{Varga_1974, title={Regulation of Melanocyte Stimulating Hormone Action at the Receptor Level: Discontinuous Binding of Hormone to Synchronized Mouse Melanoma Cells During the Cell Cycle}, volume={71}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.71.5.1590}, DOI={10.1073/pnas.71.5.1590}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Varga, J. M. and Dipasquale, A. and Pawelek, J. and McGuire, J. S. and Lerner, A. B.}, year={1974}, month=may, pages={1590–1593} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        2
      ]
    ],
    "date-time": "2025-08-02T04:17:14Z",
    "timestamp": 1754108234428
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "5",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1974,
        5
      ]
    ]
  },
  "abstract": "<jats:p>\n            Melanocyte stimulating hormone coupled to Sepharose effects an increase in tryosinase (EC 1.14.18.1; monophenol monoxygenase) activity of cultivated mouse melanoma cells. Synchronized cells are found to respond to melanocyte stimulating hormone only in the G2 phase of the cell cycle, although their response to cyclic AMP is independent of position in the cell cycle. The binding of\n            <jats:sup>125</jats:sup>\n            I-labeled melanocyte stimulating hormone occurs predominantly in G2. These observations are satisfied by a model in which the hormone can activate adenylate cyclase (EC 4.6.1.1) by binding to a melanocyte stimulating hormone receptor only in G2; the events distal to cyclic AMP production can occur throughout the cell cycle.\n          </jats:p>",
  "DOI": "10.1073/pnas.71.5.1590",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T06:55:31Z",
    "timestamp": 1149058531000
  },
  "page": "1590-1593",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 81,
  "title": "Regulation of Melanocyte Stimulating Hormone Action at the Receptor Level: Discontinuous Binding of Hormone to Synchronized Mouse Melanoma Cells During the Cell Cycle",
  "prefix": "10.1073",
  "volume": "71",
  "author": [
    {
      "given": "J. M.",
      "family": "Varga",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut 06510"
        },
        {
          "name": "Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510"
        }
      ]
    },
    {
      "given": "A.",
      "family": "Dipasquale",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut 06510"
        }
      ]
    },
    {
      "given": "J.",
      "family": "Pawelek",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut 06510"
        },
        {
          "name": "Department of Anatomy, Yale University School of Medicine, New Haven, Connecticut 06510"
        }
      ]
    },
    {
      "given": "J. S.",
      "family": "McGuire",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut 06510"
        }
      ]
    },
    {
      "given": "A. B.",
      "family": "Lerner",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut 06510"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1974,
        5
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.71.5.1590",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T14:26:00Z",
    "timestamp": 1649859960000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.71.5.1590"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1974,
        5
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          1974,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.71.5.1590"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.71.5.1590",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1974,
        5
      ]
    ]
  },
  "assertion": [
    {
      "value": "1974-05-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}