DOI: 10.1073/pnas.71.4.1174. Purification and Properties of Two RNA Polymerases from <i>Physarum polycephalum</i>

Purification and Properties of Two RNA Polymerases from Physarum polycephalum

10.1073/pnas.71.4.1174

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Two RNA polymerases have been purified from the slime mold Physarum polycephalum , one sensitive and one resistant to α-amanitin. Both enzymes are more active with denatured DNA than native DNA as a template and prefer Mn ++ rather than Mg ++ as a divalent cation. The α-amanitin-sensitive enzyme shows maximum activity at 0.15 M KCl, whereas the resistant enzyme is most active at very low ionic strength. Analysis of the resistant enzyme on polyacrylamide gels containing sodium dodecyl sulfate shows two subunits present in a 1:1 ratio with molecular weights of 205,000 and 125,000.

Bibliography

Burgess, A. B., & Burgess, R. R. (1974). Purification and Properties of Two RNA Polymerases from Physarum polycephalum. Proceedings of the National Academy of Sciences, 71(4), 1174â1177.

Authors 2

Ann Baker Burgess (first)
Richard R. Burgess (additional)

References 0 Referenced 15

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:53 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:43 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 10:57 a.m.)
Issued	51 years, 5 months ago (April 1, 1974)
Published	51 years, 5 months ago (April 1, 1974)
Published Online	51 years, 5 months ago (April 1, 1974)
Published Print	51 years, 5 months ago (April 1, 1974)

Funders 0

None

BibTeX

@article{Burgess_1974, title={Purification and Properties of Two RNA Polymerases from Physarum polycephalum}, volume={71}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.71.4.1174}, DOI={10.1073/pnas.71.4.1174}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Burgess, Ann Baker and Burgess, Richard R.}, year={1974}, month=apr, pages={1174–1177} }

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