DOI: 10.1073/pnas.71.10.4135. Kinetic Proofreading: A New Mechanism for Reducing Errors in Biosynthetic Processes Requiring High Specificity

Kinetic Proofreading: A New Mechanism for Reducing Errors in Biosynthetic Processes Requiring High Specificity

10.1073/pnas.71.10.4135

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The specificity with which the genetic code is read in protein synthesis, and with which other highly specific biosynthetic reactions take place, can be increased above the level available from free energy differences in intermediates or kinetic barriers by a process defined here as kinetic proofreading. A simple kinetic pathway is described which results in this proofreading when the reaction is strongly but nonspecifically driven, e.g., by phosphate hydrolysis. Protein synthesis, amino acid recognition, and DNA replication, all exhibit the features of this model. In each case, known reactions which otherwise appear to be useless or deleterious complications are seen to be essential to the proofreading function.

Bibliography

Hopfield, J. J. (1974). Kinetic Proofreading: A New Mechanism for Reducing Errors in Biosynthetic Processes Requiring High Specificity. Proceedings of the National Academy of Sciences, 71(10), 4135â4139.

Authors 1

J. J. Hopfield (first)

References 0 Referenced 1,237

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:48 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:40 a.m.)
Indexed	4 minutes ago (Aug. 23, 2025, 7:49 p.m.)
Issued	50 years, 10 months ago (Oct. 1, 1974)
Published	50 years, 10 months ago (Oct. 1, 1974)
Published Online	50 years, 10 months ago (Oct. 1, 1974)
Published Print	50 years, 10 months ago (Oct. 1, 1974)

Funders 0

None

BibTeX

@article{Hopfield_1974, title={Kinetic Proofreading: A New Mechanism for Reducing Errors in Biosynthetic Processes Requiring High Specificity}, volume={71}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.71.10.4135}, DOI={10.1073/pnas.71.10.4135}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hopfield, J. J.}, year={1974}, month=oct, pages={4135–4139} }

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