DOI: 10.1073/pnas.71.1.26. Molecular Complementarity of Yeast Glycoprotein Mating Factors

Molecular Complementarity of Yeast Glycoprotein Mating Factors

10.1073/pnas.71.1.26

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Cell fusion between opposite mating types 5 and 21 of the yeast Hansenula wingei is initiated by a strong sexual agglutination reaction. The mating factors responsible for the specificity of cellular recognition are complementary glycoproteins which form a physical complex in vitro . The complex is assayed by recovery of agglutination activity of the multivalent 5-factor after the univalent 21-factor has been inactivated by treatment of the complex with alkali. The 5-factor·21-factor complex, purified on Sepharose 6B, is large (several million daltons) and heterogeneous. The three peaks of 5-factor activity contain a number of combining sites proportional to molecular size.

Bibliography

Crandall, M., Lawrence, L. M., & Saunders, R. M. (1974). Molecular Complementarity of Yeast Glycoprotein Mating Factors. Proceedings of the National Academy of Sciences, 71(1), 26â29.

Authors 3

Marjorie Crandall (first)
Lawrence M. Lawrence (additional)
Robert M. Saunders (additional)

References 0 Referenced 30

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:46 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:22 a.m.)
Indexed	1 year, 9 months ago (Nov. 12, 2023, 4:15 a.m.)
Issued	51 years, 8 months ago (Jan. 1, 1974)
Published	51 years, 8 months ago (Jan. 1, 1974)
Published Online	51 years, 8 months ago (Jan. 1, 1974)
Published Print	51 years, 8 months ago (Jan. 1, 1974)

Funders 0

None

BibTeX

@article{Crandall_1974, title={Molecular Complementarity of Yeast Glycoprotein Mating Factors}, volume={71}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.71.1.26}, DOI={10.1073/pnas.71.1.26}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Crandall, Marjorie and Lawrence, Lawrence M. and Saunders, Robert M.}, year={1974}, month=jan, pages={26–29} }

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