DOI: 10.1073/pnas.71.1.172. Asymmetry in the 50S Ribosomal Subunit of <i>Escherichia coli</i>

Asymmetry in the 50S Ribosomal Subunit of Escherichia coli

10.1073/pnas.71.1.172

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A substantial separation of the centers of mass of the protein and RNA portions of the 50S ribosomal subunit has been achieved using neutron scattering. This separation reveals that the subunit has a protein-rich side, a finding which is inconsistent with many models proposed for the structure. By analyzing the variation of the radius of gyration of the subunit under conditions in which the contributions of the protein and RNA were separately enhanced by deuteration and comparing these radii to the undeuterated subunit radius, we have obtained the following values. The radius of gyration of the whole particle is 78.0 ± 0.95 Å, that of the RNA moiety is 72.5 ± 1.5 Å, and that of the protein is 73.4 ± 2.0 Å. These data give a separation of the centers of mass of the RNA and protein distributions of 57.7 ± 10 Å.

Bibliography

Moore, P. B., Engelman, D. M., & Schoenborn, B. P. (1974). Asymmetry in the 50S Ribosomal Subunit of Escherichia coli. Proceedings of the National Academy of Sciences, 71(1), 172â176.

Authors 3

P. B. Moore (first)
D. M. Engelman (additional)
B. P. Schoenborn (additional)

References 0 Referenced 34

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:46 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:22 a.m.)
Indexed	1 year, 2 months ago (June 2, 2024, 1:52 a.m.)
Issued	51 years, 7 months ago (Jan. 1, 1974)
Published	51 years, 7 months ago (Jan. 1, 1974)
Published Online	51 years, 7 months ago (Jan. 1, 1974)
Published Print	51 years, 7 months ago (Jan. 1, 1974)

Funders 0

None

BibTeX

@article{Moore_1974, title={Asymmetry in the 50S Ribosomal Subunit of Escherichia coli}, volume={71}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.71.1.172}, DOI={10.1073/pnas.71.1.172}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Moore, P. B. and Engelman, D. M. and Schoenborn, B. P.}, year={1974}, month=jan, pages={172–176} }

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