DOI: 10.1073/pnas.70.9.2610. Biosynthesis of Transfer RNA: In Vitro Conversion of Transfer RNA Precursors from Bombyx mori to 4S RNA by Escherichia coli Enzymes

Biosynthesis of Transfer RNA: In Vitro Conversion of Transfer RNA Precursors from Bombyx mori to 4S RNA by Escherichia coli Enzymes

10.1073/pnas.70.9.2610

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Evidence for the presence of rapidly labeled short-lived RNA species, presumed to be precursors to transfer RNA (tRNA), in the silk glands of Bombyx mori is presented. These precursors, which migrate between 4S and 5S markers on acrylamide gels, can be converted in vitro into molecules indistinguishable in size from tRNA. The conversion is also catalyzed by a crude enzyme fraction isolated from ribosomes of E. coli . Since a similar enzyme preparation cleaves the suppressor tRNA Tyr sequence of E. coli from its precursor molecule, it would appear that the precursor to E. coli tRNA Tyr and the precursors to silk-gland tRNAs share common structural features.

Bibliography

Chen, G. S., & Siddiqui, M. A. Q. (1973). Biosynthesis of Transfer RNA: In Vitro Conversion of Transfer RNA Precursors from Bombyx mori to 4S RNA by Escherichia coli Enzymes. Proceedings of the National Academy of Sciences, 70(9), 2610â2613.

Authors 2

G. S. Chen (first)
M. A. Q. Siddiqui (additional)

References 0 Referenced 8

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:45 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:44 a.m.)
Indexed	1 year, 11 months ago (Sept. 13, 2023, 1:30 p.m.)
Issued	51 years, 11 months ago (Sept. 1, 1973)
Published	51 years, 11 months ago (Sept. 1, 1973)
Published Online	51 years, 11 months ago (Sept. 1, 1973)
Published Print	51 years, 11 months ago (Sept. 1, 1973)

Funders 0

None

BibTeX

@article{Chen_1973, title={Biosynthesis of Transfer RNA: In Vitro Conversion of Transfer RNA Precursors from Bombyx mori to 4S RNA by Escherichia coli Enzymes}, volume={70}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.70.9.2610}, DOI={10.1073/pnas.70.9.2610}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Chen, G. S. and Siddiqui, M. A. Q.}, year={1973}, month=sep, pages={2610–2613} }

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