DOI: 10.1073/pnas.70.9.2529. Cyclic Adenosine Monophosphate Receptor: Loss of cAMP-Dependent DNA Binding Activity after Proteolysis in the Presence of Cyclic Adenosine Monophosphate

Cyclic Adenosine Monophosphate Receptor: Loss of cAMP-Dependent DNA Binding Activity after Proteolysis in the Presence of Cyclic Adenosine Monophosphate

10.1073/pnas.70.9.2529

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The cAMP receptor requires cAMP for DNA binding at pH 8.0 but shows cAMP-independent DNA binding at pH 6.0. Incubation of the cAMP receptor with proteolytic enzymes in the presence of cAMP results in loss of DNA-binding ability at pH 8, while it is still able to bind cAMP and DNA at pH 6. Incubation with proteolytic enzyme in the absence of cAMP does not affect the DNA-binding properties of the cAMP receptor. After proteolysis in the presence of cAMP, analysis by sodium dodecyl sulfateacrylamide-gel electrophoresis shows that the 22,500-dalton subunit characteristic of the untreated protein has been completely replaced by a 12,500-dalton fragment.

Bibliography

Krakow, J. S., & Pastan, I. (1973). Cyclic Adenosine Monophosphate Receptor: Loss of cAMP-Dependent DNA Binding Activity after Proteolysis in the Presence of Cyclic Adenosine Monophosphate. Proceedings of the National Academy of Sciences, 70(9), 2529â2533.

Authors 2

Joseph S. Krakow (first)
Ira Pastan (additional)

References 0 Referenced 52

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:45 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:45 a.m.)
Indexed	1 year, 7 months ago (Jan. 14, 2024, 6:24 p.m.)
Issued	52 years ago (Sept. 1, 1973)
Published	52 years ago (Sept. 1, 1973)
Published Online	52 years ago (Sept. 1, 1973)
Published Print	52 years ago (Sept. 1, 1973)

Funders 0

None

BibTeX

@article{Krakow_1973, title={Cyclic Adenosine Monophosphate Receptor: Loss of cAMP-Dependent DNA Binding Activity after Proteolysis in the Presence of Cyclic Adenosine Monophosphate}, volume={70}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.70.9.2529}, DOI={10.1073/pnas.70.9.2529}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Krakow, Joseph S. and Pastan, Ira}, year={1973}, month=sep, pages={2529–2533} }

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