DOI: 10.1073/pnas.70.8.2266. Avian Tumor Virus RNA: A Comparison of Three Sarcoma Viruses and Their Transformation-Defective Derivatives by Oligonucleotide Fingerprinting and DNA-RNA Hybridization

Avian Tumor Virus RNA: A Comparison of Three Sarcoma Viruses and Their Transformation-Defective Derivatives by Oligonucleotide Fingerprinting and DNA-RNA Hybridization

10.1073/pnas.70.8.2266

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Earlier electrophoretic analyses have shown that the 60-70S RNA of avian sarcoma viruses contains a characteristic subunit, termed class a subunit, which has a lower electrophoretic mobility than class b subunit found in transformation-defective derivatives of sarcoma viruses and in avian leukosis viruses. We have compared the RNAs of three nondefective avian sarcoma viruses, B77 and Prague and Schmidt-Ruppin strains of Rous sarcoma virus, with those of their transformation-defective ( td ) derivatives, td B77, td PR-C, and td SR-A, respectively, to determine the chemical basis for the difference between class a and b subunits. It was found by “fingerprinting” that ( 1 ) all (about 20-25) large T1 RNase-resistant oligonucleotides present in class b subunits of transformation-defective viruses have homologous counterparts in the class a subunits of corresponding nondefective sarcoma viruses and that ( 2 ) class a subunits contain a few (one or two) additional oligonucleotides that are not present in class b . By contrast the oligonucleotide fingerprints of avian tumor viruses of different strains and subgroups were very different. Cross hybridization of classes a and b RNA of sarcoma virus B77 with DNA transcribed from a corresponding transformation-defective virus td B77 showed that the two RNAs share at least 60% and differ by about 10% of their sequences. It is suggested that the structural relationship of class a and b subunits of corresponding viruses may be expressed as a = b + x , and that all the oligonucleotides present only in RNAs of sarcoma viruses but not in transformation-defective viruses of the corresponding strains are part of sequence(s) x . The possibility that x represents genetic information directly or indirectly involved in transformation of fibroblasts is discussed.

Bibliography

Lai, M. M. C., Duesberg, P. H., Horst, J., & Vogt, P. K. (1973). Avian Tumor Virus RNA: A Comparison of Three Sarcoma Viruses and Their Transformation-Defective Derivatives by Oligonucleotide Fingerprinting and DNA-RNA Hybridization. Proceedings of the National Academy of Sciences, 70(8), 2266â2270.

Authors 4

Michael M. C. Lai (first)
Peter H. Duesberg (additional)
Jürgen Horst (additional)
Peter K. Vogt (additional)

References 0 Referenced 58

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:42 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:23 a.m.)
Indexed	1 year, 2 months ago (June 15, 2024, 11:56 a.m.)
Issued	52 years, 1 month ago (Aug. 1, 1973)
Published	52 years, 1 month ago (Aug. 1, 1973)
Published Online	52 years, 1 month ago (Aug. 1, 1973)
Published Print	52 years, 1 month ago (Aug. 1, 1973)

Funders 0

None

BibTeX

@article{Lai_1973, title={Avian Tumor Virus RNA: A Comparison of Three Sarcoma Viruses and Their Transformation-Defective Derivatives by Oligonucleotide Fingerprinting and DNA-RNA Hybridization}, volume={70}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.70.8.2266}, DOI={10.1073/pnas.70.8.2266}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Lai, Michael M. C. and Duesberg, Peter H. and Horst, Jürgen and Vogt, Peter K.}, year={1973}, month=aug, pages={2266–2270} }

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  "abstract": "<jats:p>\n            Earlier electrophoretic analyses have shown that the 60-70S RNA of avian sarcoma viruses contains a characteristic subunit, termed class\n            <jats:italic>a</jats:italic>\n            subunit, which has a lower electrophoretic mobility than class\n            <jats:italic>b</jats:italic>\n            subunit found in transformation-defective derivatives of sarcoma viruses and in avian leukosis viruses. We have compared the RNAs of three nondefective avian sarcoma viruses, B77 and Prague and Schmidt-Ruppin strains of Rous sarcoma virus, with those of their transformation-defective (\n            <jats:italic>td</jats:italic>\n            ) derivatives,\n            <jats:italic>td</jats:italic>\n            B77,\n            <jats:italic>td</jats:italic>\n            PR-C, and\n            <jats:italic>td</jats:italic>\n            SR-A, respectively, to determine the chemical basis for the difference between class\n            <jats:italic>a</jats:italic>\n            and\n            <jats:italic>b</jats:italic>\n            subunits.\n          </jats:p>\n          <jats:p>\n            It was found by \u201cfingerprinting\u201d that (\n            <jats:italic>1</jats:italic>\n            ) all (about 20-25) large T1 RNase-resistant oligonucleotides present in class\n            <jats:italic>b</jats:italic>\n            subunits of transformation-defective viruses have homologous counterparts in the class\n            <jats:italic>a</jats:italic>\n            subunits of corresponding nondefective sarcoma viruses and that (\n            <jats:italic>2</jats:italic>\n            ) class\n            <jats:italic>a</jats:italic>\n            subunits contain a few (one or two) additional oligonucleotides that are not present in class\n            <jats:italic>b</jats:italic>\n            . By contrast the oligonucleotide fingerprints of avian tumor viruses of different strains and subgroups were very different.\n          </jats:p>\n          <jats:p>\n            Cross hybridization of classes\n            <jats:italic>a</jats:italic>\n            and\n            <jats:italic>b</jats:italic>\n            RNA of sarcoma virus B77 with DNA transcribed from a corresponding transformation-defective virus\n            <jats:italic>td</jats:italic>\n            B77 showed that the two RNAs share at least 60% and differ by about 10% of their sequences.\n          </jats:p>\n          <jats:p>\n            It is suggested that the structural relationship of class\n            <jats:italic>a</jats:italic>\n            and\n            <jats:italic>b</jats:italic>\n            subunits of corresponding viruses may be expressed as\n            <jats:italic>a</jats:italic>\n            =\n            <jats:italic>b</jats:italic>\n            +\n            <jats:italic>x</jats:italic>\n            , and that all the oligonucleotides present only in RNAs of sarcoma viruses but not in transformation-defective viruses of the corresponding strains are part of sequence(s)\n            <jats:italic>x</jats:italic>\n            . The possibility that\n            <jats:italic>x</jats:italic>\n            represents genetic information directly or indirectly involved in transformation of fibroblasts is discussed.\n          </jats:p>",
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