DOI: 10.1073/pnas.70.7.1968. Structure-Function Relationships in Lactate Dehydrogenase

Structure-Function Relationships in Lactate Dehydrogenase

10.1073/pnas.70.7.1968

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The binding of coenzyme and substrate are considered in relation to the known primary and tertiary structure of lactate dehydrogenase (EC 1.1.1.27). The adenine binds in a hydrophobic crevice, and the two coenzyme phosphates are oriented by interactions with the protein. The positively charged guanidinium group of arginine 101 then folds over the negatively charged phosphates, collapsing the loop region over the active center and positioning the unreactive B side of the nicotinamide in a hydrophobic protein environment. Collapse of the loop also introduces various charged groups into the vicinity of the substrate binding site. The substrate is situated between histidine 195 and the C4 position on the nicotinamide ring, and is partially oriented by interactions between its carboxyl group and arginine 171. The spatial arrangements of these groups may provide the specificity for the L-isomer of lactate.

Authors 12

Margaret J. Adams (first)
Manfred Buehner (additional)
K. Chandrasekhar (additional)
Geoffrey C. Ford (additional)
Marvin L. Hackert (additional)
Anders Liljas (additional)
Michael G. Rossmann (additional)
Ira E. Smiley (additional)
William S. Allison (additional)
Johannes Everse (additional)
Nathan O. Kaplan (additional)
Susan S. Taylor (additional)

References 0 Referenced 189

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:44 a.m.)
Indexed	18 hours, 5 minutes ago (Aug. 31, 2025, 7:20 p.m.)
Issued	52 years, 2 months ago (July 1, 1973)
Published	52 years, 2 months ago (July 1, 1973)
Published Online	52 years, 2 months ago (July 1, 1973)
Published Print	52 years, 2 months ago (July 1, 1973)

Funders 0

None

BibTeX

@article{Adams_1973, title={Structure-Function Relationships in Lactate Dehydrogenase}, volume={70}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.70.7.1968}, DOI={10.1073/pnas.70.7.1968}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Adams, Margaret J. and Buehner, Manfred and Chandrasekhar, K. and Ford, Geoffrey C. and Hackert, Marvin L. and Liljas, Anders and Rossmann, Michael G. and Smiley, Ira E. and Allison, William S. and Everse, Johannes and Kaplan, Nathan O. and Taylor, Susan S.}, year={1973}, month=jul, pages={1968–1972} }

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