DOI: 10.1073/pnas.70.3.944. Functional Correspondence Between 30S Ribosomal Proteins of <i>Escherichia coli</i> and <i>Bacillus stearothermophilus</i>

Functional Correspondence Between 30S Ribosomal Proteins of Escherichia coli and Bacillus stearothermophilus

10.1073/pnas.70.3.944

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

30S ribosomal proteins from Bacillus stearothermophilus ( B . proteins) have been fractionated and characterized with respect to their ability to replace various E. coli 30S proteins ( E. proteins) in the E. coli 30S ribosome reconstitution system. The functional counterparts of all the E . proteins, except S1, S6, S9, and S13, have been tested. In all cases, B . proteins can substitute for E . proteins. Several purified B . proteins are chemically different from their functionally homologous E . proteins. Five B . proteins are immunochemically related to E . proteins; this set includes two proteins that could not be tested in the reconstitution system (S9 and S13). Thus most, if not all, of the E . proteins have functionally equivalent counterparts among B . proteins, even though properties of the two ribosomes are different in several respects. These results suggest that the fundamental structural organization of ribosomes may be the same throughout prokaryotic organisms.

Bibliography

Higo, K., Held, W., Kahan, L., & Nomura, M. (1973). Functional Correspondence Between 30S Ribosomal Proteins of Escherichia coli and Bacillus stearothermophilus. Proceedings of the National Academy of Sciences, 70(3), 944â948.

Authors 4

K. Higo (first)
W. Held (additional)
L. Kahan (additional)
M. Nomura (additional)

References 0 Referenced 77

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:42 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:41 a.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:44 a.m.)
Issued	52 years, 5 months ago (March 1, 1973)
Published	52 years, 5 months ago (March 1, 1973)
Published Online	52 years, 5 months ago (March 1, 1973)
Published Print	52 years, 5 months ago (March 1, 1973)

Funders 0

None

BibTeX

@article{Higo_1973, title={Functional Correspondence Between 30S Ribosomal Proteins of Escherichia coli and Bacillus stearothermophilus}, volume={70}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.70.3.944}, DOI={10.1073/pnas.70.3.944}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Higo, K. and Held, W. and Kahan, L. and Nomura, M.}, year={1973}, month=mar, pages={944–948} }

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  "abstract": "<jats:p>\n            30S ribosomal proteins from\n            <jats:italic>Bacillus stearothermophilus</jats:italic>\n            (\n            <jats:italic>B</jats:italic>\n            . proteins) have been fractionated and characterized with respect to their ability to replace various\n            <jats:italic>E. coli</jats:italic>\n            30S proteins (\n            <jats:italic>E.</jats:italic>\n            proteins) in the\n            <jats:italic>E. coli</jats:italic>\n            30S ribosome reconstitution system. The functional counterparts of all the\n            <jats:italic>E</jats:italic>\n            . proteins, except S1, S6, S9, and S13, have been tested. In all cases,\n            <jats:italic>B</jats:italic>\n            . proteins can substitute for\n            <jats:italic>E</jats:italic>\n            . proteins. Several purified\n            <jats:italic>B</jats:italic>\n            . proteins are chemically different from their functionally homologous\n            <jats:italic>E</jats:italic>\n            . proteins. Five\n            <jats:italic>B</jats:italic>\n            . proteins are immunochemically related to\n            <jats:italic>E</jats:italic>\n            . proteins; this set includes two proteins that could not be tested in the reconstitution system (S9 and S13). Thus most, if not all, of the\n            <jats:italic>E</jats:italic>\n            . proteins have functionally equivalent counterparts among\n            <jats:italic>B</jats:italic>\n            . proteins, even though properties of the two ribosomes are different in several respects. These results suggest that the fundamental structural organization of ribosomes may be the same throughout prokaryotic organisms.\n          </jats:p>",
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