DOI: 10.1073/pnas.70.3.765. Microtubule Assembly in the Absence of Added Nucleotides

Microtubule Assembly in the Absence of Added Nucleotides

10.1073/pnas.70.3.765

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Microtubule assembly is enhanced by the addition of 1 M sucrose or 4 M glycerol to the reassembly mixture. Tubulin can be purified from guinea pig brain readily and in good yield by two cycles of assembly in glycerol-containing solutions. The tubules assembled in glycerol and sucrose are more stable than tubules formed in the absence of these compounds. Assembly occurs in glycerol or sucrose in the absence of ATP or GTP, but is greatly accelarated by their presence.

Bibliography

Shelanski, M. L., Gaskin, F., & Cantor, C. R. (1973). Microtubule Assembly in the Absence of Added Nucleotides. Proceedings of the National Academy of Sciences, 70(3), 765â768.

Authors 3

Michael L. Shelanski (first)
Felicia Gaskin (additional)
Charles R. Cantor (additional)

References 0 Referenced 1,346

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:42 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:41 a.m.)
Indexed	4 days, 15 hours ago (Aug. 23, 2025, 9:17 p.m.)
Issued	52 years, 5 months ago (March 1, 1973)
Published	52 years, 5 months ago (March 1, 1973)
Published Online	52 years, 5 months ago (March 1, 1973)
Published Print	52 years, 5 months ago (March 1, 1973)

Funders 0

None

BibTeX

@article{Shelanski_1973, title={Microtubule Assembly in the Absence of Added Nucleotides}, volume={70}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.70.3.765}, DOI={10.1073/pnas.70.3.765}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Shelanski, Michael L. and Gaskin, Felicia and Cantor, Charles R.}, year={1973}, month=mar, pages={765–768} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        24
      ]
    ],
    "date-time": "2025-08-24T01:17:40Z",
    "timestamp": 1755998260669
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "3",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1973,
        3
      ]
    ]
  },
  "abstract": "<jats:p>Microtubule assembly is enhanced by the addition of 1 M sucrose or 4 M glycerol to the reassembly mixture. Tubulin can be purified from guinea pig brain readily and in good yield by two cycles of assembly in glycerol-containing solutions. The tubules assembled in glycerol and sucrose are more stable than tubules formed in the absence of these compounds. Assembly occurs in glycerol or sucrose in the absence of ATP or GTP, but is greatly accelarated by their presence.</jats:p>",
  "DOI": "10.1073/pnas.70.3.765",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T06:42:45Z",
    "timestamp": 1149057765000
  },
  "page": "765-768",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 1346,
  "title": "Microtubule Assembly in the Absence of Added Nucleotides",
  "prefix": "10.1073",
  "volume": "70",
  "author": [
    {
      "given": "Michael L.",
      "family": "Shelanski",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Laboratory of Biochemical Genetics, National Heart and Lung Institute, Bethesda, Maryland 20014"
        }
      ]
    },
    {
      "given": "Felicia",
      "family": "Gaskin",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Chemistry, Columbia University, New York, N.Y. 10027"
        }
      ]
    },
    {
      "given": "Charles R.",
      "family": "Cantor",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Chemistry, Columbia University, New York, N.Y. 10027"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1973,
        3
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.70.3.765",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T14:41:33Z",
    "timestamp": 1649860893000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.70.3.765"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1973,
        3
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1973,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.70.3.765"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.70.3.765",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1973,
        3
      ]
    ]
  },
  "assertion": [
    {
      "value": "1973-03-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}