DOI: 10.1073/pnas.70.12.3626. Nucleotide Modification In Vitro of the Precursor of Transfer RNA Tyr of Escherichia coli

Nucleotide Modification In Vitro of the Precursor of Transfer RNA Tyr of Escherichia coli

10.1073/pnas.70.12.3626

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Certain nucleotides in precursor RNA of tRNA Tyr of Escherichia coli were modified in vitro with a preparation of partially purified E. coli enzyme containing ribothymidine- and pseudouridine-forming activity. The only nucleotides modified in vitro are the same as those found modified in mature tRNA. The best substrate for these modifying enzymes is the RNase P cleavage product of the precursor RNA, which contains the mature tRNA sequence. Of the two pseudouridines found in mature tRNA, one (in the TΨC sequence) can be formed in intact precursor RNA. The other (in the anticodon stem) can only be formed in the cleaved precursor RNA. The presence of modified nucleotides in the precursor RNA does not enhance its rate of cleavage by RNase P.

Bibliography

Schaefer, K. P., Altman, S., & SÃ¶ll, D. (1973). Nucleotide Modification In Vitro of the Precursor of Transfer RNA Tyr of Escherichia coli. Proceedings of the National Academy of Sciences, 70(12), 3626â3630.

Authors 3

K. P. Schaefer (first)
S. Altman (additional)
D. Söll (additional)

References 0 Referenced 41

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:47 a.m.)
Indexed	1 year, 9 months ago (Nov. 24, 2023, 1:38 a.m.)
Issued	51 years, 8 months ago (Dec. 1, 1973)
Published	51 years, 8 months ago (Dec. 1, 1973)
Published Online	51 years, 8 months ago (Dec. 1, 1973)
Published Print	51 years, 8 months ago (Dec. 1, 1973)

Funders 0

None

BibTeX

@article{Schaefer_1973, title={Nucleotide Modification In Vitro of the Precursor of Transfer RNA Tyr of Escherichia coli}, volume={70}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.70.12.3626}, DOI={10.1073/pnas.70.12.3626}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Schaefer, K. P. and Altman, S. and Söll, D.}, year={1973}, month=dec, pages={3626–3630} }

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