DOI: 10.1073/pnas.70.10.2741. Stimulation of Steroid Secretion in Adrenal Tumor Cells by Choleragen

Stimulation of Steroid Secretion in Adrenal Tumor Cells by Choleragen

10.1073/pnas.70.10.2741

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Choleragen, the pure protein from cholera toxin, stimulates steroid secretion by Y-1 adrenal tumor cells in culture. The secreted steroids are the same as seen after addition of adrenocorticotropic hormone. Half-maximal stimulation occurs at 15 pM; stimulation is essentially irreversible by washing and partially reversible (for about 1 hr) by antibody, and there is a latent period of about 60 min before stimulation is seen. Stimulation of adenylate cyclase occurs at about 30-fold higher choleragen concentrations. Gangliosides inhibit choleragen stimulation when added before but not after the toxin. Lipopolysaccharides from Escherichia coli, Salmonella typhosa , and Serratia marcescens also stimulate steroid secretion, but are less potent than choleragen.

Bibliography

Wolff, J., Temple, R., & Cook, G. H. (1973). Stimulation of Steroid Secretion in Adrenal Tumor Cells by Choleragen. Proceedings of the National Academy of Sciences, 70(10), 2741â2744.

Authors 3

J. Wolff (first)
R. Temple (additional)
G. Hope Cook (additional)

References 0 Referenced 40

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:40 a.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 4:39 p.m.)
Issued	51 years, 10 months ago (Oct. 1, 1973)
Published	51 years, 10 months ago (Oct. 1, 1973)
Published Online	51 years, 10 months ago (Oct. 1, 1973)
Published Print	51 years, 10 months ago (Oct. 1, 1973)

Funders 0

None

BibTeX

@article{Wolff_1973, title={Stimulation of Steroid Secretion in Adrenal Tumor Cells by Choleragen}, volume={70}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.70.10.2741}, DOI={10.1073/pnas.70.10.2741}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wolff, J. and Temple, R. and Cook, G. Hope}, year={1973}, month=oct, pages={2741–2744} }

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