DOI: 10.1073/pnas.69.9.2627. Regeneration of Cation-Transport Capacity in HeLa Cell Membranes After Specific Blockade by Ouabain

Regeneration of Cation-Transport Capacity in HeLa Cell Membranes After Specific Blockade by Ouabain

10.1073/pnas.69.9.2627

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The cardiac glycoside, ouabain, inhibits alkali-cation transport in HeLa cells. It binds to 0.75 × 10 6 sites per cell, and the half-time for its dissociation is 16 hr. After partial blockade by ouabain, the cell generates new ouabain-binding sites, with total restoration of transport in 10% of a cell cycle(∼3 hr). This recovery requires protein synthesis and appears to be a response to altered cell-electrolyte content, since growth of cells in media with low K + concentration enhances the titer of the transport enzyme in a fashion similar to the effect of ouabain. Totally blocked cells do not recover.

Bibliography

Vaughan, G. L., & Cook, J. S. (1972). Regeneration of Cation-Transport Capacity in HeLa Cell Membranes After Specific Blockade by Ouabain. Proceedings of the National Academy of Sciences, 69(9), 2627â2631.

Authors 2

Gerald L. Vaughan (first)
John S. Cook (additional)

References 0 Referenced 52

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:38 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:48 a.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 1:18 p.m.)
Issued	53 years ago (Sept. 1, 1972)
Published	53 years ago (Sept. 1, 1972)
Published Online	53 years ago (Sept. 1, 1972)
Published Print	53 years ago (Sept. 1, 1972)

Funders 0

None

BibTeX

@article{Vaughan_1972, title={Regeneration of Cation-Transport Capacity in HeLa Cell Membranes After Specific Blockade by Ouabain}, volume={69}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.69.9.2627}, DOI={10.1073/pnas.69.9.2627}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Vaughan, Gerald L. and Cook, John S.}, year={1972}, month=sep, pages={2627–2631} }

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