DOI: 10.1073/pnas.69.7.1702. Transcription in Yeast: Separation and Properties of Multiple RNA Polymerases

Transcription in Yeast: Separation and Properties of Multiple RNA Polymerases

10.1073/pnas.69.7.1702

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Four peaks of DNA-directed RNA polymerase activity are resolved by salt gradient elution of a sonicated yeast cell extract on DEAE-Sephadex. The enzymes, which are named IA, IB, II, and III in order of elution, all appear to come from cell nuclei. Only enzyme II is sensitive to α-amanitin. All enzymes are more active with Mn ++ than with Mg ++ as divalent ion. Enzymes IB and II have salt optima in the range 0.05-0.10 M (NH 4 ) 2 SO 4 , whereas enzyme III is maximally active at 0.20-0.25 M (NH 4 ) 2 SO 4 . With optimal salt concentration and saturating DNA, the template preference ratio, activity on native calfthymus DNA divided by activity on denatured calf-thymus DNA, is 2.2 for IB, 0.4 for II, and 3.5 for III. None of the yeast polymerases was inhibited by rifamycin SV. Rifamycin AF/013 effectively inhibited polymerases IB, II, and III.

Bibliography

Adman, R., Schultz, L. D., & Hall, B. D. (1972). Transcription in Yeast: Separation and Properties of Multiple RNA Polymerases. Proceedings of the National Academy of Sciences, 69(7), 1702â1706.

Authors 3

Ray Adman (first)
Loren D. Schultz (additional)
Benjamin D. Hall (additional)

References 0 Referenced 65

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:37 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:22 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 7:39 a.m.)
Issued	53 years, 1 month ago (July 1, 1972)
Published	53 years, 1 month ago (July 1, 1972)
Published Online	53 years, 1 month ago (July 1, 1972)
Published Print	53 years, 1 month ago (July 1, 1972)

Funders 0

None

BibTeX

@article{Adman_1972, title={Transcription in Yeast: Separation and Properties of Multiple RNA Polymerases}, volume={69}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.69.7.1702}, DOI={10.1073/pnas.69.7.1702}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Adman, Ray and Schultz, Loren D. and Hall, Benjamin D.}, year={1972}, month=jul, pages={1702–1706} }

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