DOI: 10.1073/pnas.69.5.1327. Spatial Arrangement of Ribosomal Proteins: Reaction of the <i>Escherichia coli</i> 30S Subunit with <i>bis</i> -Imidoesters

Spatial Arrangement of Ribosomal Proteins: Reaction of the Escherichia coli 30S Subunit with bis -Imidoesters

10.1073/pnas.69.5.1327

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The 30S ribosomal subunit of E. coli was treated with the bifunctional reagent bis -(methyl)suberimidate. Crosslinked ribosomal proteins were identified as bands with increased molecular weight after electrophoresis in polyacrylamide gels containing sodium dodecyl sulphate. The pattern of crosslinked products was altered when unfolded subunits were used. Free ribosomal protein was not crosslinked. Several of the crosslinked products were cleaved by ammonolysis to form the original monomeric protein constituents. The low yields of the reactions necessitated the use of radioactive proteins and auto-radiographic procedures. The crosslinked proteins were tentatively identified by coelectrophoresis of the radioactive ammonolysis products with carrier 30S protein in sodium dodecyl sulphate, and coelectrophoresis at pH 4.5 in buffers containing urea.

Bibliography

Bickle, T. A., Hershey, J. W. B., & Traut, R. R. (1972). Spatial Arrangement of Ribosomal Proteins: Reaction of the Escherichia coli 30S Subunit with bis -Imidoesters. Proceedings of the National Academy of Sciences, 69(5), 1327â1331.

Authors 3

T. A. Bickle (first)
J. W. B. Hershey (additional)
R. R. Traut (additional)

References 0 Referenced 47

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:33 a.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 4:20 p.m.)
Issued	53 years, 4 months ago (May 1, 1972)
Published	53 years, 4 months ago (May 1, 1972)
Published Online	53 years, 4 months ago (May 1, 1972)
Published Print	53 years, 4 months ago (May 1, 1972)

Funders 0

None

BibTeX

@article{Bickle_1972, title={Spatial Arrangement of Ribosomal Proteins: Reaction of the Escherichia coli 30S Subunit with bis -Imidoesters}, volume={69}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.69.5.1327}, DOI={10.1073/pnas.69.5.1327}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Bickle, T. A. and Hershey, J. W. B. and Traut, R. R.}, year={1972}, month=may, pages={1327–1331} }

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